Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3

Jiwen Li, Jin Wang, Jianxiang Wang, Zafar Nawaz, Johnson M. Liu, Jun Qin, Jiemin Wong

Research output: Contribution to journalArticle

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Abstract

We present evidence that both corepressors SMRT and N-CoR exist in large protein complexes with estimated sizes of 1.5-2 MDa in HeLa nuclear extracts. Using a combination of conventional and immunoaffinity chromatography, we have successfully isolated a SMRT complex and identified histone deacetylase 3 (HDAC3) and transducin (β)-like I (TBL1), a WD-40 repeat-containing protein, as the subunits of the purified SMRT complex. We show that the HDAC3-containing SMRT and N-CoR complexes can bind to unliganded thyroid hormone receptors (TRs) in vitro. We demonstrate further that in Xenopus oocytes, both SMRT and N-CoR also associate with HDAC3 in large protein complexes and that injection of antibodies against HDAC3 or SMRT\N-CoR led to a partial relief of repression by unliganded TR/RXR. These findings thus establish both SMRT and N-CoR complexes as bona fide HDAC-containing complexes and shed new light on the molecular pathways by which N-CoR and SMRT function in transcriptional repression.

Original languageEnglish
Pages (from-to)4342-4350
Number of pages9
JournalEMBO Journal
Volume19
Issue number16
StatePublished - Aug 15 2000
Externally publishedYes

Fingerprint

Co-Repressor Proteins
Thyroid Hormone Receptors
Proteins
Transducin
Protein Subunits
Xenopus
Chromatography
Oocytes
Injections
histone deacetylase 3
Antibodies

Keywords

  • HDAC3
  • Repression
  • SMRT and N-CoR corepressor complexes
  • TBL1

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Li, J., Wang, J., Wang, J., Nawaz, Z., Liu, J. M., Qin, J., & Wong, J. (2000). Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3. EMBO Journal, 19(16), 4342-4350.

Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3. / Li, Jiwen; Wang, Jin; Wang, Jianxiang; Nawaz, Zafar; Liu, Johnson M.; Qin, Jun; Wong, Jiemin.

In: EMBO Journal, Vol. 19, No. 16, 15.08.2000, p. 4342-4350.

Research output: Contribution to journalArticle

Li, J, Wang, J, Wang, J, Nawaz, Z, Liu, JM, Qin, J & Wong, J 2000, 'Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3', EMBO Journal, vol. 19, no. 16, pp. 4342-4350.
Li J, Wang J, Wang J, Nawaz Z, Liu JM, Qin J et al. Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3. EMBO Journal. 2000 Aug 15;19(16):4342-4350.
Li, Jiwen ; Wang, Jin ; Wang, Jianxiang ; Nawaz, Zafar ; Liu, Johnson M. ; Qin, Jun ; Wong, Jiemin. / Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3. In: EMBO Journal. 2000 ; Vol. 19, No. 16. pp. 4342-4350.
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