Bone marrow stromal cells modulate both κ light chain and Ly1 antigen expression on Ly1+ pre-B cell lines in vitro

M. G. Kruger, R. L. Riley, E. A. Riley, J. M. Elia

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Murine Ly1+ pre-B cell lines, including 70Z/3 and three pre-B cell lines derived from long-term bone marrow cultures, exhibited selective adherence to bone marrow strornal cells. In contrast, splenic B cells, the A20 B-cell lymphoma, and four Ly1- B cell lines derived from long-term bone marrow cultures failed to adhere substantially to bone marrow stroma. Ly1+ pre-B cell lines were induced to express κ light chains by exposure to either lipopolysaccharide (LPS). recombinant interleukin-1 (IL-1), or stromal cells. However, induction of κ light chains failed to prevent pre-B cell adherence to stromal cells. Supernatants derived from primary bone marrow stromal cells decreased Ly1 expression on the Ly1+ pre-B cell lines. These experiments suggest that (1) expression of immunoglobulin light chains by developing Ly1+ pre-B cells is mediated by bone marrow stromal cells; (2) loss of specific adherence to stroma is progressive and occurs post-light chain induction; and (3) soluble products of stromal cells may downregulate expression of surface Ly1 on otherwise Ly1+ pre-B cells. The importance of these observations to the development of both the Ly1- and Ly1+ B cell lineages in the mouse is discussed.

Original languageEnglish (US)
Pages (from-to)383-392
Number of pages10
JournalBlood
Volume76
Issue number2
DOIs
StatePublished - 1990

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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