Bone marrow stromal cell-mediated tissue sparing enhances functional repair after spinal cord contusion in adult rats

Gaby J. Ritfeld, Rishi D S Nandoe Tewarie, Katarina Vajn, Sahar T. Rahiem, Andres Hurtado, Dane F. Wendell, Raymund A C Roos, Martin Oudega

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Bone marrow stromal cell (BMSC) transplantation has shown promise for repair of the spinal cord. We showed earlier that a BMSC transplant limits the loss of spinal nervous tissue after a contusive injury. Here, we addressed the premise that BMSC-mediated tissue sparing underlies functional recovery in adult rats after a contusion of the thoracic spinal cord. Our results reveal that after 2 months BMSCs had elicited a significant increase in spared tissue volumes and in blood vessel density in the contusion epicenter. A strong functional relationship existed between spared tissue volumes and blood vessel density. BMSC-transplanted rats exhibited significant improvements in motor, sensorimotor, and sensory functions, which were strongly correlated with spared tissue volumes. Retrograde tracing revealed that rats with BMSCs had twice as many descending brainstem neurons with an axon projecting beyond the contused spinal cord segment and these correlated strongly with the improved motor/sensorimotor functions but not sensory functions. Together, our data indicate that tissue sparing greatly contributes to BMSC-mediated functional repair after spinal cord contusion. The preservation/formation of blood vessels and sparing/regeneration of descending brainstem axons may be important mediators of the BMSC-mediated anatomical and functional improvements.

Original languageEnglish (US)
Pages (from-to)1561-1575
Number of pages15
JournalCell Transplantation
Volume21
Issue number7
DOIs
StatePublished - 2012
Externally publishedYes

Fingerprint

Mesenchymal Stromal Cells
Spinal Cord Injuries
Rats
Bone
Repair
Tissue
Blood vessels
Blood Vessels
Contusions
Brain Stem
Axons
Spinal Cord Regeneration
Nerve Tissue
Transplants
Bone Marrow Transplantation
Neurons
Regeneration
Spinal Cord
Thorax
Recovery

Keywords

  • Allodynia
  • Bone marrow stromal cell (BMSC)
  • Gridwalk
  • Locomotion
  • Neuroprotection
  • Transplantation

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation
  • Biomedical Engineering

Cite this

Bone marrow stromal cell-mediated tissue sparing enhances functional repair after spinal cord contusion in adult rats. / Ritfeld, Gaby J.; Nandoe Tewarie, Rishi D S; Vajn, Katarina; Rahiem, Sahar T.; Hurtado, Andres; Wendell, Dane F.; Roos, Raymund A C; Oudega, Martin.

In: Cell Transplantation, Vol. 21, No. 7, 2012, p. 1561-1575.

Research output: Contribution to journalArticle

Ritfeld, GJ, Nandoe Tewarie, RDS, Vajn, K, Rahiem, ST, Hurtado, A, Wendell, DF, Roos, RAC & Oudega, M 2012, 'Bone marrow stromal cell-mediated tissue sparing enhances functional repair after spinal cord contusion in adult rats', Cell Transplantation, vol. 21, no. 7, pp. 1561-1575. https://doi.org/10.3727/096368912X640484
Ritfeld, Gaby J. ; Nandoe Tewarie, Rishi D S ; Vajn, Katarina ; Rahiem, Sahar T. ; Hurtado, Andres ; Wendell, Dane F. ; Roos, Raymund A C ; Oudega, Martin. / Bone marrow stromal cell-mediated tissue sparing enhances functional repair after spinal cord contusion in adult rats. In: Cell Transplantation. 2012 ; Vol. 21, No. 7. pp. 1561-1575.
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