Bone marrow mononuclear cell therapy led to alveolar-capillary membrane repair, improving lung mechanics in endotoxin-induced acute lung injury

Luiz Felipe M. Prota, Roberta Soares, Tatiana Maron-Gutierrez, Raquel C. Castiglione, Cristiane Souza Baez Garcia, Maria Cristina Ebole Santana, Jackson Souza-Menezes, Soraia C. Abreu, Vivian Samoto, Marcelo Felipe Santiago, Vera Luiza Capelozzi, Christina Maeda Takiya, Patricia R.M. Rocco, Marcelo M. Morales

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The aim of this study was to test the hypothesis that bone marrow mononuclear cell (BMDMC) therapy led an improvement in lung mechanics and histology in endotoxin-induced lung injury. Twenty-four C57BL/6 mice were randomly divided into four groups (n = 6 each). In the acute lung injur;y (ALI) group, Escherichia coli lipopolysaccharide (LPS) was instilled intratracheally (40 μg, IT), and control (C) mice received saline (0.05 ml, IT). One hour after the administration of saline or LPS, BMDMC (2 × 107 cells) was intravenously injected. At day 28, animals were anesthetized and lung mechanics [static elastance (Est), resistive (ΔP1), and viscoelastic (ΔP2) pressures] and histology (light and electron microscopy) were analyzed. Immunogold electron microscopy was used to evaluate if multinucleate cells were type II epithelial cells. BMDMC therapy prevented endotoxin-induced lung inflammation, alveolar collapse, and interstitial edema. In addition, BMDMC administration led to epithelial and endothelial repair with multinucleated type II pneumocytes. These histological changes yielded a reduction in lung Est, ΔP1, and ΔP2 compared to ALI. In the present experimental ALI model, the administration of BMDMC yielded a reduction in the inflammatory process and a repair of epithelium and endothelium, reducing the amount of alveolar collapse, thus leading to an improvement in lung mechanics.

Original languageEnglish (US)
Pages (from-to)965-971
Number of pages7
JournalCell Transplantation
Volume19
Issue number8
DOIs
StatePublished - Nov 22 2010
Externally publishedYes

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Acute Lung Injury
Cell- and Tissue-Based Therapy
Mechanics
Endotoxins
Bone Marrow Cells
Bone
Histology
Repair
Membranes
Lung
Electron microscopy
Lipopolysaccharides
Electron Microscopy
Escherichia coli
Optical microscopy
Animals
Alveolar Epithelial Cells
Lung Injury
Inbred C57BL Mouse
Endothelium

Keywords

  • Bone marrow-derived mononuclear cells (BMDMC)
  • Electron microscopy
  • Inflammation
  • Lung mechanics

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

Cite this

Prota, L. F. M., Soares, R., Maron-Gutierrez, T., Castiglione, R. C., Baez Garcia, C. S., Ebole Santana, M. C., ... Morales, M. M. (2010). Bone marrow mononuclear cell therapy led to alveolar-capillary membrane repair, improving lung mechanics in endotoxin-induced acute lung injury. Cell Transplantation, 19(8), 965-971. https://doi.org/10.3727/096368910X506845

Bone marrow mononuclear cell therapy led to alveolar-capillary membrane repair, improving lung mechanics in endotoxin-induced acute lung injury. / Prota, Luiz Felipe M.; Soares, Roberta; Maron-Gutierrez, Tatiana; Castiglione, Raquel C.; Baez Garcia, Cristiane Souza; Ebole Santana, Maria Cristina; Souza-Menezes, Jackson; Abreu, Soraia C.; Samoto, Vivian; Santiago, Marcelo Felipe; Capelozzi, Vera Luiza; Takiya, Christina Maeda; Rocco, Patricia R.M.; Morales, Marcelo M.

In: Cell Transplantation, Vol. 19, No. 8, 22.11.2010, p. 965-971.

Research output: Contribution to journalArticle

Prota, LFM, Soares, R, Maron-Gutierrez, T, Castiglione, RC, Baez Garcia, CS, Ebole Santana, MC, Souza-Menezes, J, Abreu, SC, Samoto, V, Santiago, MF, Capelozzi, VL, Takiya, CM, Rocco, PRM & Morales, MM 2010, 'Bone marrow mononuclear cell therapy led to alveolar-capillary membrane repair, improving lung mechanics in endotoxin-induced acute lung injury', Cell Transplantation, vol. 19, no. 8, pp. 965-971. https://doi.org/10.3727/096368910X506845
Prota, Luiz Felipe M. ; Soares, Roberta ; Maron-Gutierrez, Tatiana ; Castiglione, Raquel C. ; Baez Garcia, Cristiane Souza ; Ebole Santana, Maria Cristina ; Souza-Menezes, Jackson ; Abreu, Soraia C. ; Samoto, Vivian ; Santiago, Marcelo Felipe ; Capelozzi, Vera Luiza ; Takiya, Christina Maeda ; Rocco, Patricia R.M. ; Morales, Marcelo M. / Bone marrow mononuclear cell therapy led to alveolar-capillary membrane repair, improving lung mechanics in endotoxin-induced acute lung injury. In: Cell Transplantation. 2010 ; Vol. 19, No. 8. pp. 965-971.
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