Bone marrow mesenchymal stem cell-derived cd63+ exosomes transport wnt3a exteriorly and enhance dermal fibroblast proliferation, migration, and angiogenesis in vitro

Jeffrey D. McBride, Luis Rodriguez-Menocal, Wellington Guzman, Ambar Candanedo, Marta Garcia-Contreras, Evangelos V Badiavas

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Wnts are secreted glycoproteins that regulate stem cell self-renewal, differentiation, and cell-To-cell communication during embryonic development and in adult tissues. Bone marrow mesenchymal stem cells (BM-MSCs) have been shown to stimulate dermis repair and regeneration; however, it is unclear how BM-MSCs may modulate downstream Wnt signaling. While recent reports implicate that Wnt ligands and Wnt messenger RNAs (such as Wnt4) exist within the interior compartment of exosomes, it has been debated whether or not Wnts exist on the exterior surface of exosomes to travel in the extracellular space. To help answer this question, we utilized flow cytometry of magnetic beads coated with anti-CD63 antibodies and found, for the first time, that Wnt3a protein is detectable exteriorly on CD63+ exosomes derived from BM-MSCs over-secreting Wnt3a into serum-free conditioned media (Wnt3a CM). Our data suggest that CD63+ exosomes significantly help transport exterior Wnt3a signal to recipient cells to promote fibroblast and endothelial functions. During purification of exosomes, we unexpectedly found that use of ultracentrifugation alone significantly decreased the ability to detect exteriorly bound Wnt3a on CD63+ exosomes, however, polyethylene glycol (PEG)-mediated exosome-enrichment before exosome-purification (with ultracentrifugation into a sucrose cushion) resulted in exosomes more likely to retain exterior Wnt3a detectability and downstream Wnt/beta-catenin activity. Our findings indicate the important role that purification methods may have on stem cell-derived Wnt-exosome activity in downstream assays. The ability for BM-MSC Wnt3a CM and exosomes to stimulate dermal fibroblast proliferation and migration, and endothelial angiogenesis in vitro, was significantly decreased after CD63+-exosome depletion or knockdown of Wnt coreceptor LRP6 in recipient cells, suggesting both are required for optimal Wnt-exosome activity in our system. Thus, BM-MSC-derived CD63+ exosomes are a significant carrier of exterior Wnt3a within high Wnt environments, resulting in downstream fibroblast proliferation, migration, and angiogenesis in vitro.

Original languageEnglish (US)
Pages (from-to)1384-1398
Number of pages15
JournalStem Cells and Development
Volume26
Issue number19
DOIs
StatePublished - Oct 1 2017

Fingerprint

Exosomes
Mesenchymal Stromal Cells
Fibroblasts
Bone Marrow
Skin
Ultracentrifugation
In Vitro Techniques
Wnt3A Protein
Serum-Free Culture Media
Extracellular Space
beta Catenin
Dermis
Conditioned Culture Medium
Cell Communication

Keywords

  • Angiogenesis
  • Bone marrow mesenchymal stem cell
  • Exosome
  • Fibroblast
  • Wnt3a

ASJC Scopus subject areas

  • Hematology
  • Developmental Biology
  • Cell Biology

Cite this

Bone marrow mesenchymal stem cell-derived cd63+ exosomes transport wnt3a exteriorly and enhance dermal fibroblast proliferation, migration, and angiogenesis in vitro. / McBride, Jeffrey D.; Rodriguez-Menocal, Luis; Guzman, Wellington; Candanedo, Ambar; Garcia-Contreras, Marta; Badiavas, Evangelos V.

In: Stem Cells and Development, Vol. 26, No. 19, 01.10.2017, p. 1384-1398.

Research output: Contribution to journalArticle

McBride, Jeffrey D. ; Rodriguez-Menocal, Luis ; Guzman, Wellington ; Candanedo, Ambar ; Garcia-Contreras, Marta ; Badiavas, Evangelos V. / Bone marrow mesenchymal stem cell-derived cd63+ exosomes transport wnt3a exteriorly and enhance dermal fibroblast proliferation, migration, and angiogenesis in vitro. In: Stem Cells and Development. 2017 ; Vol. 26, No. 19. pp. 1384-1398.
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