Bone marrow-generated dendritic cells pulsed with tumor extracts or tumor RNA induce antitumor immunity against central nervous system tumors

David M. Ashley, Brenda Faiola, Smita Nair, Laura P. Hale, Darell D. Bigner, Eli Gilboa

Research output: Contribution to journalArticle

379 Scopus citations

Abstract

Recent studies have shown that the brain is not a barrier to successful active immunotherapy that uses gene-modified autologous tumor cell vaccines. In this study, we compared the efficacy of two types of vaccines for the treatment of tumors within the central nervous system (CNS): dendritic cell (DC)-based vaccines pulsed with either tumor extract or tumor RNA, and cytokine gene-modified tumor vaccines. Using the B16/F10 murine melanoma (B16) as a model for CNS tumor, we show that vaccination with bone marrow- generated DCs, pulsed with either B16 cell extract or B16 total RNA, can induce specific cytotoxic T lymphocytes against B16 tumor cells. Both types of DC vaccines were able to protect animals from tumors located in the CNS. DC-based vaccines also led to prolonged survival in mice with tumors placed before the initiation of vaccine therapy. The DC-based vaccines were at least as effective, if not more so, as vaccines containing B16 tumor cells in which the granulocytic macrophage colony-stimulating factor gene had been modified. These data support the use of DCbased vaccines for the treatment of patients with CNS tumors.

Original languageEnglish (US)
Pages (from-to)1177-1182
Number of pages6
JournalJournal of Experimental Medicine
Volume186
Issue number7
DOIs
StatePublished - Oct 6 1997

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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