Bone marrow cytoplasmic deoxyribonucleic acid polymerase. Variation of pH and ionic environment as a possible control mechanism

John Byrnes, Kathleen M. Downey, Antero G. So

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A cytoplasmic DNA polymerase has been purified 200fold from erythroid hyperplastic bone marrow. This enzyme shows an absolute requirement for a divalent cation, which may be either Mg2+ or Mn2+, and is stimulated about fourfold by a monovalent cation, NH4+ or K+. The enzyme preferentially uses activated calf thymus DNA and poly[d(A-T)] as a template, while native calf thymus DNA and synthetic DNA/RNA hybrids are rather poor templates. DNA synthesis with this enzyme is inhibited by low concentrations of heme or ethidium bromide. The rate of DNA synthesis with the cytoplasmic DNA polymerase is markedly dependent on pH as well as the concentrations of divalent and monovalent cations and the effects of these factors are interdependent and interrelated. Changes in pH have profound effects on the concentration of monovalent and divalent cations required for optimal activity and, conversely, changes in the concentrations of divalent and monovalent cations also influence the optimal pH of the reaction. The mechanism of salt activation has been shown to involve a dissociation of the DNA polymerase from an 11,6-S dimer to an 8-S monomer.

Original languageEnglish
Pages (from-to)4378-4384
Number of pages7
JournalBiochemistry
Volume12
Issue number22
StatePublished - Dec 1 1973

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Monovalent Cations
Divalent Cations
Bone
DNA-Directed DNA Polymerase
Bone Marrow
DNA
Enzymes
Ethidium
Heme
Dimers
Salts
Monomers
Chemical activation
RNA
calf thymus DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Bone marrow cytoplasmic deoxyribonucleic acid polymerase. Variation of pH and ionic environment as a possible control mechanism. / Byrnes, John; Downey, Kathleen M.; So, Antero G.

In: Biochemistry, Vol. 12, No. 22, 01.12.1973, p. 4378-4384.

Research output: Contribution to journalArticle

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