Bone marrow augmentation of donor-cell chimerism in kidney, liver, heart, and pancreas islet transplantation

Paulo Fontes, Abdul S. Rao, Anthony J. Demetris, Adriana Zeevi, Massimo Trucco, Pat Carroll, Witold Rybka, William A. Rudert, Camillo Ricordi, Forrest Dodson, Ron Shapiro, Andreas Tzakis, Satoru Todo, Kareem Abu-Elmagd, Mark Jordan, John J. Fung, Camillo Ricordi

Research output: Contribution to journalArticle

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Abstract

We have previously postulated that donor cell chimerism in organ transplantation is needed to attain a tolerant state. Here we show that donor cell chimerism can be augmented in organ recipients if they are infused perioperatively with 3 x 108 per kg of unmodified donor bone marrow cells and are kept on a conventional immunosuppressive regimen of tacrolimus (FK506) and prednisolone. 36 patients took part, of whom the first 18 patients have good transplanted kidney (n = 10), liver (n = 7), and heart (n = 7) function when followed up between 4 and 16 months. All patients are well. We found persistent multilineage leucocyte chimerism in blood of 17 recipients by flow cytometry and PCR techniques to detect donor alleles or Y chromosomes in female recipients of male organs. The use of the 5-antigen HLA matched same sex donor precluded detection of chimerism in one patient.

Original languageEnglish
Pages (from-to)151-155
Number of pages5
JournalLancet
Volume344
Issue number8916
DOIs
StatePublished - Jul 21 1994

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Pancreas Transplantation
Islets of Langerhans Transplantation
Chimerism
Bone Marrow
Tissue Donors
Kidney
Liver
Tacrolimus
Y Chromosome
Organ Transplantation
Immunosuppressive Agents
HLA Antigens
Prednisolone
Bone Marrow Cells
Flow Cytometry
Leukocytes
Alleles
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Medicine(all)

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Bone marrow augmentation of donor-cell chimerism in kidney, liver, heart, and pancreas islet transplantation. / Fontes, Paulo; Rao, Abdul S.; Demetris, Anthony J.; Zeevi, Adriana; Trucco, Massimo; Carroll, Pat; Rybka, Witold; Rudert, William A.; Ricordi, Camillo; Dodson, Forrest; Shapiro, Ron; Tzakis, Andreas; Todo, Satoru; Abu-Elmagd, Kareem; Jordan, Mark; Fung, John J.; Ricordi, Camillo.

In: Lancet, Vol. 344, No. 8916, 21.07.1994, p. 151-155.

Research output: Contribution to journalArticle

Fontes, P, Rao, AS, Demetris, AJ, Zeevi, A, Trucco, M, Carroll, P, Rybka, W, Rudert, WA, Ricordi, C, Dodson, F, Shapiro, R, Tzakis, A, Todo, S, Abu-Elmagd, K, Jordan, M, Fung, JJ & Ricordi, C 1994, 'Bone marrow augmentation of donor-cell chimerism in kidney, liver, heart, and pancreas islet transplantation', Lancet, vol. 344, no. 8916, pp. 151-155. https://doi.org/10.1016/S0140-6736(94)92756-1
Fontes, Paulo ; Rao, Abdul S. ; Demetris, Anthony J. ; Zeevi, Adriana ; Trucco, Massimo ; Carroll, Pat ; Rybka, Witold ; Rudert, William A. ; Ricordi, Camillo ; Dodson, Forrest ; Shapiro, Ron ; Tzakis, Andreas ; Todo, Satoru ; Abu-Elmagd, Kareem ; Jordan, Mark ; Fung, John J. ; Ricordi, Camillo. / Bone marrow augmentation of donor-cell chimerism in kidney, liver, heart, and pancreas islet transplantation. In: Lancet. 1994 ; Vol. 344, No. 8916. pp. 151-155.
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