Bombesin may stimulate proliferation of human pancreatic cancer cells through an autocrine pathway

Qiming J. Wang, Joseph A. Knezetic, Andrew V Schally, Parviz M. Pour, Thomas E. Adrian

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Bombesin is trophic to normal pancreas and acinar cell adenocarcinoma, but its effects on ductal cell tumors are undetermined. The autocrine growth effects of bombesin on well-differentiated (HPAF, CD11) and poorly differentiated (CD18, PANC-1) human ductal pancreatic cancer cell lines were investigated. Receptor binding of labeled bombesin was measured in a whole-cell microplate assay. Bombesin production was measured by radioimmunoassay. Proliferative responses were quantified using the MTT assay. Messenger RNA for bombesin and its receptor were identified by primer extension analysis. A single class of high-affinity binding sites was detected on HPAF and CD18 cells. Similar affinities and high receptor densities were found on the 2 cell lines. Bombesin was secreted by all 4 cell lines during 24-hr culture in serum-free media, and its recovery was enhanced in the presence- of protease inhibitors. Primer extension analysis demonstrated the presence of mRNA for both bombesin and its receptor in HPAF, CD18, CD11 and PANC-1 cells, even though no functional receptor war found in the latter 2 lines. Bombesin significantly stimulated the proliferation of HPAF and CD18 cells. This trophic effect was inhibited by the specific bombesin antagonist RC-3095. Bombesin may act as an autocrine growth factor in some human pancreatic cancer cell lines. Furthermore, other cell lines transcribe mRNA for bombesin receptors but have no functional bombesin receptors, suggesting a genetic or post-translational change in the receptor for these cells. Bombesin may be involved as a growth factor in the development of pancreatic ductal adenocarcinoma in humans. This possible autocrine growth pathway may provide an avenue for therapeutic intervention in this malignant disease.

Original languageEnglish
Pages (from-to)528-534
Number of pages7
JournalInternational Journal of Cancer
Volume68
Issue number4
DOIs
StatePublished - Dec 21 1996
Externally publishedYes

Fingerprint

Bombesin
Pancreatic Neoplasms
Bombesin Receptors
Cell Line
Messenger RNA
Intercellular Signaling Peptides and Proteins
Acinar Cell Carcinoma
Serum-Free Culture Media
Growth
Protease Inhibitors
Radioimmunoassay
Pancreas
Adenocarcinoma
Binding Sites

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Bombesin may stimulate proliferation of human pancreatic cancer cells through an autocrine pathway. / Wang, Qiming J.; Knezetic, Joseph A.; Schally, Andrew V; Pour, Parviz M.; Adrian, Thomas E.

In: International Journal of Cancer, Vol. 68, No. 4, 21.12.1996, p. 528-534.

Research output: Contribution to journalArticle

Wang, Qiming J. ; Knezetic, Joseph A. ; Schally, Andrew V ; Pour, Parviz M. ; Adrian, Thomas E. / Bombesin may stimulate proliferation of human pancreatic cancer cells through an autocrine pathway. In: International Journal of Cancer. 1996 ; Vol. 68, No. 4. pp. 528-534.
@article{ee1ac3eecad84998820f005b7e2b4ce7,
title = "Bombesin may stimulate proliferation of human pancreatic cancer cells through an autocrine pathway",
abstract = "Bombesin is trophic to normal pancreas and acinar cell adenocarcinoma, but its effects on ductal cell tumors are undetermined. The autocrine growth effects of bombesin on well-differentiated (HPAF, CD11) and poorly differentiated (CD18, PANC-1) human ductal pancreatic cancer cell lines were investigated. Receptor binding of labeled bombesin was measured in a whole-cell microplate assay. Bombesin production was measured by radioimmunoassay. Proliferative responses were quantified using the MTT assay. Messenger RNA for bombesin and its receptor were identified by primer extension analysis. A single class of high-affinity binding sites was detected on HPAF and CD18 cells. Similar affinities and high receptor densities were found on the 2 cell lines. Bombesin was secreted by all 4 cell lines during 24-hr culture in serum-free media, and its recovery was enhanced in the presence- of protease inhibitors. Primer extension analysis demonstrated the presence of mRNA for both bombesin and its receptor in HPAF, CD18, CD11 and PANC-1 cells, even though no functional receptor war found in the latter 2 lines. Bombesin significantly stimulated the proliferation of HPAF and CD18 cells. This trophic effect was inhibited by the specific bombesin antagonist RC-3095. Bombesin may act as an autocrine growth factor in some human pancreatic cancer cell lines. Furthermore, other cell lines transcribe mRNA for bombesin receptors but have no functional bombesin receptors, suggesting a genetic or post-translational change in the receptor for these cells. Bombesin may be involved as a growth factor in the development of pancreatic ductal adenocarcinoma in humans. This possible autocrine growth pathway may provide an avenue for therapeutic intervention in this malignant disease.",
author = "Wang, {Qiming J.} and Knezetic, {Joseph A.} and Schally, {Andrew V} and Pour, {Parviz M.} and Adrian, {Thomas E.}",
year = "1996",
month = "12",
day = "21",
doi = "10.1002/(SICI)1097-0215(19961115)68:4<528::AID-IJC20>3.0.CO;2-#",
language = "English",
volume = "68",
pages = "528--534",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Bombesin may stimulate proliferation of human pancreatic cancer cells through an autocrine pathway

AU - Wang, Qiming J.

AU - Knezetic, Joseph A.

AU - Schally, Andrew V

AU - Pour, Parviz M.

AU - Adrian, Thomas E.

PY - 1996/12/21

Y1 - 1996/12/21

N2 - Bombesin is trophic to normal pancreas and acinar cell adenocarcinoma, but its effects on ductal cell tumors are undetermined. The autocrine growth effects of bombesin on well-differentiated (HPAF, CD11) and poorly differentiated (CD18, PANC-1) human ductal pancreatic cancer cell lines were investigated. Receptor binding of labeled bombesin was measured in a whole-cell microplate assay. Bombesin production was measured by radioimmunoassay. Proliferative responses were quantified using the MTT assay. Messenger RNA for bombesin and its receptor were identified by primer extension analysis. A single class of high-affinity binding sites was detected on HPAF and CD18 cells. Similar affinities and high receptor densities were found on the 2 cell lines. Bombesin was secreted by all 4 cell lines during 24-hr culture in serum-free media, and its recovery was enhanced in the presence- of protease inhibitors. Primer extension analysis demonstrated the presence of mRNA for both bombesin and its receptor in HPAF, CD18, CD11 and PANC-1 cells, even though no functional receptor war found in the latter 2 lines. Bombesin significantly stimulated the proliferation of HPAF and CD18 cells. This trophic effect was inhibited by the specific bombesin antagonist RC-3095. Bombesin may act as an autocrine growth factor in some human pancreatic cancer cell lines. Furthermore, other cell lines transcribe mRNA for bombesin receptors but have no functional bombesin receptors, suggesting a genetic or post-translational change in the receptor for these cells. Bombesin may be involved as a growth factor in the development of pancreatic ductal adenocarcinoma in humans. This possible autocrine growth pathway may provide an avenue for therapeutic intervention in this malignant disease.

AB - Bombesin is trophic to normal pancreas and acinar cell adenocarcinoma, but its effects on ductal cell tumors are undetermined. The autocrine growth effects of bombesin on well-differentiated (HPAF, CD11) and poorly differentiated (CD18, PANC-1) human ductal pancreatic cancer cell lines were investigated. Receptor binding of labeled bombesin was measured in a whole-cell microplate assay. Bombesin production was measured by radioimmunoassay. Proliferative responses were quantified using the MTT assay. Messenger RNA for bombesin and its receptor were identified by primer extension analysis. A single class of high-affinity binding sites was detected on HPAF and CD18 cells. Similar affinities and high receptor densities were found on the 2 cell lines. Bombesin was secreted by all 4 cell lines during 24-hr culture in serum-free media, and its recovery was enhanced in the presence- of protease inhibitors. Primer extension analysis demonstrated the presence of mRNA for both bombesin and its receptor in HPAF, CD18, CD11 and PANC-1 cells, even though no functional receptor war found in the latter 2 lines. Bombesin significantly stimulated the proliferation of HPAF and CD18 cells. This trophic effect was inhibited by the specific bombesin antagonist RC-3095. Bombesin may act as an autocrine growth factor in some human pancreatic cancer cell lines. Furthermore, other cell lines transcribe mRNA for bombesin receptors but have no functional bombesin receptors, suggesting a genetic or post-translational change in the receptor for these cells. Bombesin may be involved as a growth factor in the development of pancreatic ductal adenocarcinoma in humans. This possible autocrine growth pathway may provide an avenue for therapeutic intervention in this malignant disease.

UR - http://www.scopus.com/inward/record.url?scp=0029808418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029808418&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0215(19961115)68:4<528::AID-IJC20>3.0.CO;2-#

DO - 10.1002/(SICI)1097-0215(19961115)68:4<528::AID-IJC20>3.0.CO;2-#

M3 - Article

C2 - 8945626

AN - SCOPUS:0029808418

VL - 68

SP - 528

EP - 534

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 4

ER -