Bombesin induces a reduction of somatostatin inhibition of adenylyl cyclase activity, G(i) function, and somatostatin receptors in rat exocrine pancreas

I. Álvaro-Alonso, G. Muñoz-Acedo, E. Rodríguez-Martín, Andrew V Schally, E. Arilla

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To analyze the effect of bombesin on the somatostatin (SS) mechanism of action in the exocrine pancreas, male Wistar rats (250-270 g) were injected intraperitoneally with bombesin (10 μg/kg) three times daily at 8-h intervals for 7 or 14 days. Bombesin attenuated the ability of SS to inhibit forskolin-stimulated adenylyl cyclase activity in pancreatic acinar membranes. However, it did not decrease the ability of forskolin to stimulate the adenylyl cyclase catalytic subunit. The ability of 5'-guanylylimidodiphosphate [Gpp(NH)p] (a nonhydrolyzable GTP analog) to inhibit forskolin-stimulated adenylyl cyclase activity was diminished in pancreatic acinar cell membranes from bombesin-treated rats. Bombesin administration did not affect the ADP-ribosylation of a 41-kDa G protein catalyzed by pertussis toxin. The maximal SS binding capacity of pancreatic acinar membranes from bombesin-treated rats was decreased when compared with controls at the two time periods studied. The bombesin/gastrin-releasing peptide antagonist [D-Tpi6,Leu13ψ(CH2NH)Leu14]bombesin (6-14) (RC-3095) (10 μg/kg ip), injected three times daily at 8-h intervals for 7 or 14 days, had a similar effect to that of bombesin on the SS mechanism of action. The combined administration of bombesin and its antagonist RC-3095 had a greater effect on the SS receptor-effector system than when administered separately. The present study indicates that the pancreatic SS receptor-effector system may be regulated by bombesin in vivo. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)723-730
Number of pages8
JournalPeptides
Volume20
Issue number6
DOIs
StatePublished - Aug 1 1999
Externally publishedYes

Fingerprint

Bombesin
Exocrine Pancreas
Somatostatin Receptors
Somatostatin
Adenylyl Cyclases
Rats
Colforsin
Gastrin-Releasing Peptide
Guanylyl Imidodiphosphate
Membranes
Acinar Cells
Pertussis Toxin
Cell membranes
Guanosine Triphosphate
GTP-Binding Proteins
Adenosine Diphosphate
Wistar Rats
Catalytic Domain
Cell Membrane

Keywords

  • Adenylyl cyclase
  • Bombesin
  • G(i) protein
  • Proglumide
  • RC-3095
  • Somatostatin receptor

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Bombesin induces a reduction of somatostatin inhibition of adenylyl cyclase activity, G(i) function, and somatostatin receptors in rat exocrine pancreas. / Álvaro-Alonso, I.; Muñoz-Acedo, G.; Rodríguez-Martín, E.; Schally, Andrew V; Arilla, E.

In: Peptides, Vol. 20, No. 6, 01.08.1999, p. 723-730.

Research output: Contribution to journalArticle

Álvaro-Alonso, I. ; Muñoz-Acedo, G. ; Rodríguez-Martín, E. ; Schally, Andrew V ; Arilla, E. / Bombesin induces a reduction of somatostatin inhibition of adenylyl cyclase activity, G(i) function, and somatostatin receptors in rat exocrine pancreas. In: Peptides. 1999 ; Vol. 20, No. 6. pp. 723-730.
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AU - Schally, Andrew V

AU - Arilla, E.

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