BMP signaling initiates a neural crest differentiation program in embryonic rat CNS stem cells

Shyam Gajavelli; Patrick M. Wood; Diane Pennica; Scott R. Whittemore; Pantelis Tsoulfas

doi:10.1016/j.expneurol.2004.03.026

BMP signaling initiates a neural crest differentiation program in embryonic rat CNS stem cells

Shyam Gajavelli, Patrick M. Wood, Diane Pennica, Scott R. Whittemore, Pantelis Tsoulfas

Neurological Surgery

Research output: Contribution to journal › Article

28 Scopus citations

Abstract

Bone morphogenetic proteins (BMPs) have an important role in neuronal and astrocytic differentiation of embryonic and adult neural stem cells (NSCs). Here, we show that BMP6, BMP7, GDF5, and GDF6 instructively differentiate E12, E14, and E17 rat cortical NSCs into a variety of neural crest lineages. Clonal analysis shows that BMP7-treated NSCs develop mostly into smooth muscle and peripheral glia. We observed a rapid induction of premigratory neural crest markers like p75^NTR, and AP-2 alpha followed by Msx1, Msx2, and Slug, transcription factors that participate in neural crest development. These results suggest that NSCs cultured in vitro in the presence of FGF2 display expanded developmental potential.

Original language	English (US)
Pages (from-to)	205-223
Number of pages	19
Journal	Experimental neurology
Volume	188
Issue number	2
DOIs	https://doi.org/10.1016/j.expneurol.2004.03.026
State	Published - Aug 1 2004

Keywords

Bone morphogenetic proteins
Cardiotrophin-1
Glial fibrillary acidic protein
Neural crest
Neural stem cells
p21
Peripheral glia
Smooth muscle
Smooth muscle actin
Sox10

ASJC Scopus subject areas

Neurology
Neuroscience(all)

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Gajavelli, S., Wood, P. M., Pennica, D., Whittemore, S. R., & Tsoulfas, P. (2004). BMP signaling initiates a neural crest differentiation program in embryonic rat CNS stem cells. Experimental neurology, 188(2), 205-223. https://doi.org/10.1016/j.expneurol.2004.03.026

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abstract = "Bone morphogenetic proteins (BMPs) have an important role in neuronal and astrocytic differentiation of embryonic and adult neural stem cells (NSCs). Here, we show that BMP6, BMP7, GDF5, and GDF6 instructively differentiate E12, E14, and E17 rat cortical NSCs into a variety of neural crest lineages. Clonal analysis shows that BMP7-treated NSCs develop mostly into smooth muscle and peripheral glia. We observed a rapid induction of premigratory neural crest markers like p75NTR, and AP-2 alpha followed by Msx1, Msx2, and Slug, transcription factors that participate in neural crest development. These results suggest that NSCs cultured in vitro in the presence of FGF2 display expanded developmental potential.",

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