Abstract
Bone morphogenetic proteins (BMPs) have an important role in neuronal and astrocytic differentiation of embryonic and adult neural stem cells (NSCs). Here, we show that BMP6, BMP7, GDF5, and GDF6 instructively differentiate E12, E14, and E17 rat cortical NSCs into a variety of neural crest lineages. Clonal analysis shows that BMP7-treated NSCs develop mostly into smooth muscle and peripheral glia. We observed a rapid induction of premigratory neural crest markers like p75NTR, and AP-2 alpha followed by Msx1, Msx2, and Slug, transcription factors that participate in neural crest development. These results suggest that NSCs cultured in vitro in the presence of FGF2 display expanded developmental potential.
| Original language | English |
|---|---|
| Pages (from-to) | 205-223 |
| Number of pages | 19 |
| Journal | Experimental Neurology |
| Volume | 188 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 1 2004 |
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Keywords
- Bone morphogenetic proteins
- Cardiotrophin-1
- Glial fibrillary acidic protein
- Neural crest
- Neural stem cells
- p21
- Peripheral glia
- Smooth muscle
- Smooth muscle actin
- Sox10
ASJC Scopus subject areas
- Neurology
- Neuroscience(all)
Cite this
BMP signaling initiates a neural crest differentiation program in embryonic rat CNS stem cells. / Gajavelli, Shyam; Wood, Patrick; Pennica, Diane; Whittemore, Scott R.; Tsoulfas, Pantelis.
In: Experimental Neurology, Vol. 188, No. 2, 01.08.2004, p. 205-223.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - BMP signaling initiates a neural crest differentiation program in embryonic rat CNS stem cells
AU - Gajavelli, Shyam
AU - Wood, Patrick
AU - Pennica, Diane
AU - Whittemore, Scott R.
AU - Tsoulfas, Pantelis
PY - 2004/8/1
Y1 - 2004/8/1
N2 - Bone morphogenetic proteins (BMPs) have an important role in neuronal and astrocytic differentiation of embryonic and adult neural stem cells (NSCs). Here, we show that BMP6, BMP7, GDF5, and GDF6 instructively differentiate E12, E14, and E17 rat cortical NSCs into a variety of neural crest lineages. Clonal analysis shows that BMP7-treated NSCs develop mostly into smooth muscle and peripheral glia. We observed a rapid induction of premigratory neural crest markers like p75NTR, and AP-2 alpha followed by Msx1, Msx2, and Slug, transcription factors that participate in neural crest development. These results suggest that NSCs cultured in vitro in the presence of FGF2 display expanded developmental potential.
AB - Bone morphogenetic proteins (BMPs) have an important role in neuronal and astrocytic differentiation of embryonic and adult neural stem cells (NSCs). Here, we show that BMP6, BMP7, GDF5, and GDF6 instructively differentiate E12, E14, and E17 rat cortical NSCs into a variety of neural crest lineages. Clonal analysis shows that BMP7-treated NSCs develop mostly into smooth muscle and peripheral glia. We observed a rapid induction of premigratory neural crest markers like p75NTR, and AP-2 alpha followed by Msx1, Msx2, and Slug, transcription factors that participate in neural crest development. These results suggest that NSCs cultured in vitro in the presence of FGF2 display expanded developmental potential.
KW - Bone morphogenetic proteins
KW - Cardiotrophin-1
KW - Glial fibrillary acidic protein
KW - Neural crest
KW - Neural stem cells
KW - p21
KW - Peripheral glia
KW - Smooth muscle
KW - Smooth muscle actin
KW - Sox10
UR - http://www.scopus.com/inward/record.url?scp=3042706273&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042706273&partnerID=8YFLogxK
U2 - 10.1016/j.expneurol.2004.03.026
DO - 10.1016/j.expneurol.2004.03.026
M3 - Article
C2 - 15246821
AN - SCOPUS:3042706273
VL - 188
SP - 205
EP - 223
JO - Experimental Neurology
JF - Experimental Neurology
SN - 0014-4886
IS - 2
ER -