@article{2863430fa173491d915e04e382c36118,
title = "BMP signaling initiates a neural crest differentiation program in embryonic rat CNS stem cells",
abstract = "Bone morphogenetic proteins (BMPs) have an important role in neuronal and astrocytic differentiation of embryonic and adult neural stem cells (NSCs). Here, we show that BMP6, BMP7, GDF5, and GDF6 instructively differentiate E12, E14, and E17 rat cortical NSCs into a variety of neural crest lineages. Clonal analysis shows that BMP7-treated NSCs develop mostly into smooth muscle and peripheral glia. We observed a rapid induction of premigratory neural crest markers like p75NTR, and AP-2 alpha followed by Msx1, Msx2, and Slug, transcription factors that participate in neural crest development. These results suggest that NSCs cultured in vitro in the presence of FGF2 display expanded developmental potential.",
keywords = "Bone morphogenetic proteins, Cardiotrophin-1, Glial fibrillary acidic protein, Neural crest, Neural stem cells, p21, Peripheral glia, Smooth muscle, Smooth muscle actin, Sox10",
author = "Shyam Gajavelli and Wood, {Patrick M.} and Diane Pennica and Whittemore, {Scott R.} and Pantelis Tsoulfas",
note = "Funding Information: This project has been funded in part with federal funds from the National Institute of Neurological Disorders and Stroke, National Institutes of Health, under Contract N01-NS-6-2349, additional support from The Miami Project to Cure Paralysis, The FaBene Foundation, Wilson Foundation and Abramson Foundation (P.T). Commonwealth of Kentucky Research Challenge for excellence NS38665, RR15576 (SRW). The authors thank Linda White, Yelena Pressman and Anna Gomez for antibodies and Schwann cell cultures, Peggy Bates and Professor Mary Bunge for electron microscopy. Huang Yu, Larry Dishaw, Andrew J Darr and Juan C Baez for technical assistance, Dan Castellanos for statistics. Vempati Uma Devi, Kristine de La Torre, and Barbara Myers for critical reading of the manuscript, Diana Masella for assisting in preparing the manuscript.",
year = "2004",
month = aug,
doi = "10.1016/j.expneurol.2004.03.026",
language = "English (US)",
volume = "188",
pages = "205--223",
journal = "Experimental Neurology",
issn = "0014-4886",
publisher = "Academic Press Inc.",
number = "2",
}