BMP signaling initiates a neural crest differentiation program in embryonic rat CNS stem cells

Shyam Gajavelli, Patrick M. Wood, Diane Pennica, Scott R. Whittemore, Pantelis Tsoulfas

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Bone morphogenetic proteins (BMPs) have an important role in neuronal and astrocytic differentiation of embryonic and adult neural stem cells (NSCs). Here, we show that BMP6, BMP7, GDF5, and GDF6 instructively differentiate E12, E14, and E17 rat cortical NSCs into a variety of neural crest lineages. Clonal analysis shows that BMP7-treated NSCs develop mostly into smooth muscle and peripheral glia. We observed a rapid induction of premigratory neural crest markers like p75NTR, and AP-2 alpha followed by Msx1, Msx2, and Slug, transcription factors that participate in neural crest development. These results suggest that NSCs cultured in vitro in the presence of FGF2 display expanded developmental potential.

Original languageEnglish (US)
Pages (from-to)205-223
Number of pages19
JournalExperimental neurology
Volume188
Issue number2
DOIs
StatePublished - Aug 1 2004

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Keywords

  • Bone morphogenetic proteins
  • Cardiotrophin-1
  • Glial fibrillary acidic protein
  • Neural crest
  • Neural stem cells
  • p21
  • Peripheral glia
  • Smooth muscle
  • Smooth muscle actin
  • Sox10

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

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