BMP signaling initiates a neural crest differentiation program in embryonic rat CNS stem cells

Shyam Gajavelli; Patrick M. Wood; Diane Pennica; Scott R. Whittemore; Pantelis Tsoulfas

doi:10.1016/j.expneurol.2004.03.026

BMP signaling initiates a neural crest differentiation program in embryonic rat CNS stem cells

Shyam Gajavelli, Patrick M. Wood, Diane Pennica, Scott R. Whittemore, Pantelis Tsoulfas

Neurological Surgery

Research output: Contribution to journal › Article

28 Scopus citations

Abstract

Bone morphogenetic proteins (BMPs) have an important role in neuronal and astrocytic differentiation of embryonic and adult neural stem cells (NSCs). Here, we show that BMP6, BMP7, GDF5, and GDF6 instructively differentiate E12, E14, and E17 rat cortical NSCs into a variety of neural crest lineages. Clonal analysis shows that BMP7-treated NSCs develop mostly into smooth muscle and peripheral glia. We observed a rapid induction of premigratory neural crest markers like p75^NTR, and AP-2 alpha followed by Msx1, Msx2, and Slug, transcription factors that participate in neural crest development. These results suggest that NSCs cultured in vitro in the presence of FGF2 display expanded developmental potential.

Original language	English (US)
Pages (from-to)	205-223
Number of pages	19
Journal	Experimental neurology
Volume	188
Issue number	2
DOIs	https://doi.org/10.1016/j.expneurol.2004.03.026
State	Published - Aug 1 2004

Fingerprint

Keywords

Bone morphogenetic proteins
Cardiotrophin-1
Glial fibrillary acidic protein
Neural crest
Neural stem cells
p21
Peripheral glia
Smooth muscle
Smooth muscle actin
Sox10

ASJC Scopus subject areas

Neurology
Neuroscience(all)

Cite this

Gajavelli, S., Wood, P. M., Pennica, D., Whittemore, S. R., & Tsoulfas, P. (2004). BMP signaling initiates a neural crest differentiation program in embryonic rat CNS stem cells. Experimental neurology, 188(2), 205-223. https://doi.org/10.1016/j.expneurol.2004.03.026

@article{2863430fa173491d915e04e382c36118,

title = "BMP signaling initiates a neural crest differentiation program in embryonic rat CNS stem cells",

abstract = "Bone morphogenetic proteins (BMPs) have an important role in neuronal and astrocytic differentiation of embryonic and adult neural stem cells (NSCs). Here, we show that BMP6, BMP7, GDF5, and GDF6 instructively differentiate E12, E14, and E17 rat cortical NSCs into a variety of neural crest lineages. Clonal analysis shows that BMP7-treated NSCs develop mostly into smooth muscle and peripheral glia. We observed a rapid induction of premigratory neural crest markers like p75NTR, and AP-2 alpha followed by Msx1, Msx2, and Slug, transcription factors that participate in neural crest development. These results suggest that NSCs cultured in vitro in the presence of FGF2 display expanded developmental potential.",

keywords = "Bone morphogenetic proteins, Cardiotrophin-1, Glial fibrillary acidic protein, Neural crest, Neural stem cells, p21, Peripheral glia, Smooth muscle, Smooth muscle actin, Sox10",

author = "Shyam Gajavelli and Wood, {Patrick M.} and Diane Pennica and Whittemore, {Scott R.} and Pantelis Tsoulfas",

year = "2004",

month = aug

day = "1",

doi = "10.1016/j.expneurol.2004.03.026",

language = "English (US)",

volume = "188",

pages = "205--223",

journal = "Experimental Neurology",

issn = "0014-4886",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - BMP signaling initiates a neural crest differentiation program in embryonic rat CNS stem cells

AU - Gajavelli, Shyam

AU - Wood, Patrick M.

AU - Pennica, Diane

AU - Whittemore, Scott R.

AU - Tsoulfas, Pantelis

PY - 2004/8/1

Y1 - 2004/8/1

N2 - Bone morphogenetic proteins (BMPs) have an important role in neuronal and astrocytic differentiation of embryonic and adult neural stem cells (NSCs). Here, we show that BMP6, BMP7, GDF5, and GDF6 instructively differentiate E12, E14, and E17 rat cortical NSCs into a variety of neural crest lineages. Clonal analysis shows that BMP7-treated NSCs develop mostly into smooth muscle and peripheral glia. We observed a rapid induction of premigratory neural crest markers like p75NTR, and AP-2 alpha followed by Msx1, Msx2, and Slug, transcription factors that participate in neural crest development. These results suggest that NSCs cultured in vitro in the presence of FGF2 display expanded developmental potential.

AB - Bone morphogenetic proteins (BMPs) have an important role in neuronal and astrocytic differentiation of embryonic and adult neural stem cells (NSCs). Here, we show that BMP6, BMP7, GDF5, and GDF6 instructively differentiate E12, E14, and E17 rat cortical NSCs into a variety of neural crest lineages. Clonal analysis shows that BMP7-treated NSCs develop mostly into smooth muscle and peripheral glia. We observed a rapid induction of premigratory neural crest markers like p75NTR, and AP-2 alpha followed by Msx1, Msx2, and Slug, transcription factors that participate in neural crest development. These results suggest that NSCs cultured in vitro in the presence of FGF2 display expanded developmental potential.

KW - Bone morphogenetic proteins

KW - Cardiotrophin-1

KW - Glial fibrillary acidic protein

KW - Neural crest

KW - Neural stem cells

KW - p21

KW - Peripheral glia

KW - Smooth muscle

KW - Smooth muscle actin

KW - Sox10

UR - http://www.scopus.com/inward/record.url?scp=3042706273&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042706273&partnerID=8YFLogxK

U2 - 10.1016/j.expneurol.2004.03.026

DO - 10.1016/j.expneurol.2004.03.026

M3 - Article

C2 - 15246821

AN - SCOPUS:3042706273

VL - 188

SP - 205

EP - 223

JO - Experimental Neurology

JF - Experimental Neurology

SN - 0014-4886

IS - 2

ER -

Access to Document

10.1016/j.expneurol.2004.03.026