Blood acid-base regulation during environmental hyperoxia in the rainbow trout (Salmo gairdneri)

Chris M. Wood, Eric B. Jackson

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Blood acid-base balance, blood gases, respiration, ventilation, and renal function were studied in the rainbow trout during and following sustained environmental hyperoxia (PiO2 = 350-650 Torr). Animals were chronically fitted with dorsal aortic cannulae for repititive blood sampling, oral membranes for the measurement of ventilation, and bladder catheters for continuous urine collection. Hyperoxia caused a proportional increase in arterial O2 tension and a stable 60% reduction in ventilation volume (V̇w), the latter mainly due to a decrease in ventilatory stroke volume. O2 consumption exhibited a short-term elevation. Arterial CO2 tension (PaCO2) rose within 1 h, causing an immediate drop in arterial pH (pHa), and continued to increase gradually thereafter, reaching a value 2-4x the normoxic control level after 96-192 h. Compensation of the associated acidosis by the accumulation of [HCO3-] in the blood plasma started within 5-6 h, and was complete by 48 h. Thereafter, further compensation occurred simultaneously with the gradual rise in PaCO2. The kidney played an important active role in this compensation by preventing excretion of the accumulated [HCO3-]. Upon reinstitution of normoxia, PaCO2 dropped to control levels within 1 h, and restoration of blood acid-base status by reduction of [HCO3-] had commenced by this time. A complete return to contrl values occurred within 20 h. During hyperoxia, an experimental elevation of the depressed V̇w above normoxic levels caused only a minor and transient reduction in PaCO2 and no change in pHa, but injection of branchial vasidilator 1-isoprenaline (10 μmol/kg) produced a large drop in PaCO2 and rise in pHa. It is concluded that the rise in PaCO2 during hyperoxia is mainly due to internal diffusive and/or perfusive limitation associated with branchial vasoconstriction, rather than to external convective limitation associated with the decreased V̇w.

Original languageEnglish (US)
Pages (from-to)351-372
Number of pages22
JournalRespiration Physiology
Volume42
Issue number3
DOIs
StatePublished - Dec 1980

Keywords

  • Acid-base balance
  • Environmental hyperoxia
  • Isoprenaline
  • Renal function
  • Salmo gairdneri
  • Ventilation

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine

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