An injection of 1 ml of a rabbit anti luteinizing hormone releasing hormone (LH RH) serum at 9 AM on the day of proestrus prevented the usual preovulatory surge of LH and follicle stimulating hormone (FSH) and blocked ovulation in cycling female rats. Injection of 1 ml of normal rabbit serum did not prevent the preovulatory surge of LH and FSH and did not block ovulation. The results suggest that antiserum to the LH RH decapeptide, exogenously administered, neutralized endogenous LH RH and thereby prevented the preovulatory surge of gonadotropin release. The LH RH decapeptide may represent the physiological FSH RH, as well as the LH RH, or alternately LH RH and FSH RH may both possess a common antigenic determinant, Leu Arg Pro Gly NH2, to the anti LH RH serum used in this experiment.
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