Bleomycin-induced Chromosomal Aberrations in Cultured Mammalian Cells

Awtar Krishan

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Mouse fibroblasts (L-929) and human cells (HeLa and CCRF-CEM lymphoblasts) exposed to bleomycin in vitro for 20 hr showed a marked inhibition in the number of cells entering mitosis. Bleomycin-induced chromosomal aberrations were seen in cells from the three cultured lines and were mostly in the form of chromatid gaps, breaks, and fragments, and multiradial translocations. Human lymphoblasts (CCRF-CEM) were more resistant to the bleomycin-induced mitotic inhibition and chromosomal aberrations than the L-929 or HeLa cells. A small number of chromatid fragments and translocations were also seen in cells exposed to bleomycin in the G2 or post-DNA-synthetic part of the cell cycle.

Original languageEnglish (US)
Pages (from-to)961-965
Number of pages5
JournalCancer Research
Volume33
Issue number5
StatePublished - May 1973

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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