Biphasic effect of estradiol benzoate on the pituitary responsiveness to LH RH

J. A. Vilchez Martinez, A. Arimura, L. Debeljuk, Andrew V Schally

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Pituitary responses to LH releasing hormone (LH RH) was studied in normal diestrous rats 2, 4, 6, 9, 14, 24, and 48 hr after injection of estradiol benzoate (EB). A dose of 0.4 μg LH RH always raised serum LH in the oil treated rats. The magnitude of the increase varied according to the stage of the estrous cycle, being greatest in proestrus, and smallest between the evening of the day of diestrus I and the morning of the day of diestrus II. Two to 6 hr after pretreatment with EB, injection of this dose of LH RH failed to induce a significant rise of serum LH. At 9 hr, the same dose of LH RH did increase serum LH, the magnitude of the response being the same as that found in the oil treated control rats. By 14-24 hr, EB had augmented the pituitary response to LH RH. At 48 hr, the magnitude of the rise of serum LH after LH RH in EB treated rats was like that found at 24 hr, but this high responsiveness to LH RH apparently can not be attributed to the EB since a similar responsiveness was seen in the respective oil treated group. The basal levels of LH in the oil treated rats were lowest at the evening of diestrus I and in the morning of diestrus II. On the other hand, pretreatment with EB lowered the basal LH levels 2-24 hr later. From these results, it is evident that in rats EB, at the dose used, exhibited a biphasic effect of the pituitary response to LH RH, the early effect being inhibitory and the later effect being stimulatory.

Original languageEnglish
Pages (from-to)1300-1303
Number of pages4
JournalEndocrinology
Volume94
Issue number5
StatePublished - Dec 1 1974
Externally publishedYes

Fingerprint

Gonadotropin-Releasing Hormone
Diestrus
Oils
Serum
Proestrus
Injections
Estrous Cycle
estradiol 3-benzoate

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Vilchez Martinez, J. A., Arimura, A., Debeljuk, L., & Schally, A. V. (1974). Biphasic effect of estradiol benzoate on the pituitary responsiveness to LH RH. Endocrinology, 94(5), 1300-1303.

Biphasic effect of estradiol benzoate on the pituitary responsiveness to LH RH. / Vilchez Martinez, J. A.; Arimura, A.; Debeljuk, L.; Schally, Andrew V.

In: Endocrinology, Vol. 94, No. 5, 01.12.1974, p. 1300-1303.

Research output: Contribution to journalArticle

Vilchez Martinez, JA, Arimura, A, Debeljuk, L & Schally, AV 1974, 'Biphasic effect of estradiol benzoate on the pituitary responsiveness to LH RH', Endocrinology, vol. 94, no. 5, pp. 1300-1303.
Vilchez Martinez JA, Arimura A, Debeljuk L, Schally AV. Biphasic effect of estradiol benzoate on the pituitary responsiveness to LH RH. Endocrinology. 1974 Dec 1;94(5):1300-1303.
Vilchez Martinez, J. A. ; Arimura, A. ; Debeljuk, L. ; Schally, Andrew V. / Biphasic effect of estradiol benzoate on the pituitary responsiveness to LH RH. In: Endocrinology. 1974 ; Vol. 94, No. 5. pp. 1300-1303.
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