Abstract
Pituitary responses to LH releasing hormone (LH RH) was studied in normal diestrous rats 2, 4, 6, 9, 14, 24, and 48 hr after injection of estradiol benzoate (EB). A dose of 0.4 μg LH RH always raised serum LH in the oil treated rats. The magnitude of the increase varied according to the stage of the estrous cycle, being greatest in proestrus, and smallest between the evening of the day of diestrus I and the morning of the day of diestrus II. Two to 6 hr after pretreatment with EB, injection of this dose of LH RH failed to induce a significant rise of serum LH. At 9 hr, the same dose of LH RH did increase serum LH, the magnitude of the response being the same as that found in the oil treated control rats. By 14-24 hr, EB had augmented the pituitary response to LH RH. At 48 hr, the magnitude of the rise of serum LH after LH RH in EB treated rats was like that found at 24 hr, but this high responsiveness to LH RH apparently can not be attributed to the EB since a similar responsiveness was seen in the respective oil treated group. The basal levels of LH in the oil treated rats were lowest at the evening of diestrus I and in the morning of diestrus II. On the other hand, pretreatment with EB lowered the basal LH levels 2-24 hr later. From these results, it is evident that in rats EB, at the dose used, exhibited a biphasic effect of the pituitary response to LH RH, the early effect being inhibitory and the later effect being stimulatory.
| Original language | English |
|---|---|
| Pages (from-to) | 1300-1303 |
| Number of pages | 4 |
| Journal | Endocrinology |
| Volume | 94 |
| Issue number | 5 |
| State | Published - Dec 1 1974 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
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Biphasic effect of estradiol benzoate on the pituitary responsiveness to LH RH. / Vilchez Martinez, J. A.; Arimura, A.; Debeljuk, L.; Schally, Andrew V.
In: Endocrinology, Vol. 94, No. 5, 01.12.1974, p. 1300-1303.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biphasic effect of estradiol benzoate on the pituitary responsiveness to LH RH
AU - Vilchez Martinez, J. A.
AU - Arimura, A.
AU - Debeljuk, L.
AU - Schally, Andrew V
PY - 1974/12/1
Y1 - 1974/12/1
N2 - Pituitary responses to LH releasing hormone (LH RH) was studied in normal diestrous rats 2, 4, 6, 9, 14, 24, and 48 hr after injection of estradiol benzoate (EB). A dose of 0.4 μg LH RH always raised serum LH in the oil treated rats. The magnitude of the increase varied according to the stage of the estrous cycle, being greatest in proestrus, and smallest between the evening of the day of diestrus I and the morning of the day of diestrus II. Two to 6 hr after pretreatment with EB, injection of this dose of LH RH failed to induce a significant rise of serum LH. At 9 hr, the same dose of LH RH did increase serum LH, the magnitude of the response being the same as that found in the oil treated control rats. By 14-24 hr, EB had augmented the pituitary response to LH RH. At 48 hr, the magnitude of the rise of serum LH after LH RH in EB treated rats was like that found at 24 hr, but this high responsiveness to LH RH apparently can not be attributed to the EB since a similar responsiveness was seen in the respective oil treated group. The basal levels of LH in the oil treated rats were lowest at the evening of diestrus I and in the morning of diestrus II. On the other hand, pretreatment with EB lowered the basal LH levels 2-24 hr later. From these results, it is evident that in rats EB, at the dose used, exhibited a biphasic effect of the pituitary response to LH RH, the early effect being inhibitory and the later effect being stimulatory.
AB - Pituitary responses to LH releasing hormone (LH RH) was studied in normal diestrous rats 2, 4, 6, 9, 14, 24, and 48 hr after injection of estradiol benzoate (EB). A dose of 0.4 μg LH RH always raised serum LH in the oil treated rats. The magnitude of the increase varied according to the stage of the estrous cycle, being greatest in proestrus, and smallest between the evening of the day of diestrus I and the morning of the day of diestrus II. Two to 6 hr after pretreatment with EB, injection of this dose of LH RH failed to induce a significant rise of serum LH. At 9 hr, the same dose of LH RH did increase serum LH, the magnitude of the response being the same as that found in the oil treated control rats. By 14-24 hr, EB had augmented the pituitary response to LH RH. At 48 hr, the magnitude of the rise of serum LH after LH RH in EB treated rats was like that found at 24 hr, but this high responsiveness to LH RH apparently can not be attributed to the EB since a similar responsiveness was seen in the respective oil treated group. The basal levels of LH in the oil treated rats were lowest at the evening of diestrus I and in the morning of diestrus II. On the other hand, pretreatment with EB lowered the basal LH levels 2-24 hr later. From these results, it is evident that in rats EB, at the dose used, exhibited a biphasic effect of the pituitary response to LH RH, the early effect being inhibitory and the later effect being stimulatory.
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UR - http://www.scopus.com/inward/citedby.url?scp=0016212497&partnerID=8YFLogxK
M3 - Article
C2 - 4595981
AN - SCOPUS:0016212497
VL - 94
SP - 1300
EP - 1303
JO - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 5
ER -