Biotin-Modified Surfaces by Electrochemical Polymerization of Biotinyl-Tyramide

Seung Tae Yang, Allan Witkowski, Richard S. Hutchins, Donna L. Scott, Leonidas G. Bachas

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Poly(biotinyl-tyramide) films were created by the electrochemical oxidation of biotinyl-tyramide. Electropolymerization in basic solutions such as NaOH/methanol or aqueous NaOH led to adherent poly(biotinyl) films on both glassy-carbon (GC) and indium(tin) oxide (ITO) electrodes. Cyclic voltammetry revealed that the resulting poly(biotinyl-tyramide) films passivated the electrode surface. Studies using an avidin-β-galactosidase conjugate showed that the films provide biotin sites capable of selectively binding avidin.

Original languageEnglish (US)
Pages (from-to)58-60
Number of pages3
JournalElectroanalysis
Volume10
Issue number1
DOIs
StatePublished - Jan 1998
Externally publishedYes

Keywords

  • Biotinyl-tyramide
  • Cyclic voltammetry
  • Electrochemical polymerization
  • Glassy carbon electrode
  • Indium(tin)oxide electrode
  • Selectively binding avidin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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