Biotin-Modified Surfaces by Electrochemical Polymerization of Biotinyl-Tyramide

Seung Tae Yang; Allan Witkowski; Richard S. Hutchins; Donna L. Scott; Leonidas G. Bachas

doi:10.1002/(sici)1521-4109(199801)10:1<58::aid-elan58>3.0.co;2-z

Biotin-Modified Surfaces by Electrochemical Polymerization of Biotinyl-Tyramide

Seung Tae Yang, Allan Witkowski, Richard S. Hutchins, Donna L. Scott, Leonidas G. Bachas

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Poly(biotinyl-tyramide) films were created by the electrochemical oxidation of biotinyl-tyramide. Electropolymerization in basic solutions such as NaOH/methanol or aqueous NaOH led to adherent poly(biotinyl) films on both glassy-carbon (GC) and indium(tin) oxide (ITO) electrodes. Cyclic voltammetry revealed that the resulting poly(biotinyl-tyramide) films passivated the electrode surface. Studies using an avidin-β-galactosidase conjugate showed that the films provide biotin sites capable of selectively binding avidin.

Original language	English (US)
Pages (from-to)	58-60
Number of pages	3
Journal	Electroanalysis
Volume	10
Issue number	1
DOIs	https://doi.org/10.1002/(sici)1521-4109(199801)10:1<58::aid-elan58>3.0.co;2-z
State	Published - Jan 1998
Externally published	Yes

Keywords

Biotinyl-tyramide
Cyclic voltammetry
Electrochemical polymerization
Glassy carbon electrode
Indium(tin)oxide electrode
Selectively binding avidin

ASJC Scopus subject areas

Analytical Chemistry
Electrochemistry

Access to Document

10.1002/(sici)1521-4109(199801)10:1<58::aid-elan58>3.0.co;2-z

Cite this

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title = "Biotin-Modified Surfaces by Electrochemical Polymerization of Biotinyl-Tyramide",

abstract = "Poly(biotinyl-tyramide) films were created by the electrochemical oxidation of biotinyl-tyramide. Electropolymerization in basic solutions such as NaOH/methanol or aqueous NaOH led to adherent poly(biotinyl) films on both glassy-carbon (GC) and indium(tin) oxide (ITO) electrodes. Cyclic voltammetry revealed that the resulting poly(biotinyl-tyramide) films passivated the electrode surface. Studies using an avidin-β-galactosidase conjugate showed that the films provide biotin sites capable of selectively binding avidin.",

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T1 - Biotin-Modified Surfaces by Electrochemical Polymerization of Biotinyl-Tyramide

AU - Yang, Seung Tae

AU - Witkowski, Allan

AU - Hutchins, Richard S.

AU - Scott, Donna L.

AU - Bachas, Leonidas G.

PY - 1998/1

Y1 - 1998/1

N2 - Poly(biotinyl-tyramide) films were created by the electrochemical oxidation of biotinyl-tyramide. Electropolymerization in basic solutions such as NaOH/methanol or aqueous NaOH led to adherent poly(biotinyl) films on both glassy-carbon (GC) and indium(tin) oxide (ITO) electrodes. Cyclic voltammetry revealed that the resulting poly(biotinyl-tyramide) films passivated the electrode surface. Studies using an avidin-β-galactosidase conjugate showed that the films provide biotin sites capable of selectively binding avidin.

AB - Poly(biotinyl-tyramide) films were created by the electrochemical oxidation of biotinyl-tyramide. Electropolymerization in basic solutions such as NaOH/methanol or aqueous NaOH led to adherent poly(biotinyl) films on both glassy-carbon (GC) and indium(tin) oxide (ITO) electrodes. Cyclic voltammetry revealed that the resulting poly(biotinyl-tyramide) films passivated the electrode surface. Studies using an avidin-β-galactosidase conjugate showed that the films provide biotin sites capable of selectively binding avidin.

KW - Biotinyl-tyramide

KW - Cyclic voltammetry

KW - Electrochemical polymerization

KW - Glassy carbon electrode

KW - Indium(tin)oxide electrode

KW - Selectively binding avidin

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Biotin-Modified Surfaces by Electrochemical Polymerization of Biotinyl-Tyramide

Abstract

Keywords

ASJC Scopus subject areas

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