Biotin-Modified Surfaces by Electrochemical Polymerization of Biotinyl-Tyramide

Seung T. Yang, Allan Witkowski, Richard S. Hutchins, Donna L. Scott, Leonidas G Bachas

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Poly(biotinyl-tyramide) films were created by the electrochemical oxidation of biotinyl-tyramide. Electropolymerization in basic solutions such as NaOH/methanol or aqueous NaOH led to adherent poly(biotinyl) films on both glassy-carbon (GC) and indium(tin) oxide (ITO) electrodes. Cyclic voltammetry revealed that the resulting poly(biotinyl-tyramide) films passivated the electrode surface. Studies using an avidin-β-galactosidase conjugate showed that the films provide biotin sites capable of selectively binding avidin.

Original languageEnglish (US)
Pages (from-to)58-60
Number of pages3
JournalElectroanalysis
Volume10
Issue number1
StatePublished - Jan 1998
Externally publishedYes

Fingerprint

Electropolymerization
Biotin
Avidin
Galactosidases
Electrodes
Electrochemical oxidation
Glassy carbon
Tin oxides
Indium
Cyclic voltammetry
Methanol

Keywords

  • Biotinyl-tyramide
  • Cyclic voltammetry
  • Electrochemical polymerization
  • Glassy carbon electrode
  • Indium(tin)oxide electrode
  • Selectively binding avidin

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Yang, S. T., Witkowski, A., Hutchins, R. S., Scott, D. L., & Bachas, L. G. (1998). Biotin-Modified Surfaces by Electrochemical Polymerization of Biotinyl-Tyramide. Electroanalysis, 10(1), 58-60.

Biotin-Modified Surfaces by Electrochemical Polymerization of Biotinyl-Tyramide. / Yang, Seung T.; Witkowski, Allan; Hutchins, Richard S.; Scott, Donna L.; Bachas, Leonidas G.

In: Electroanalysis, Vol. 10, No. 1, 01.1998, p. 58-60.

Research output: Contribution to journalArticle

Yang, ST, Witkowski, A, Hutchins, RS, Scott, DL & Bachas, LG 1998, 'Biotin-Modified Surfaces by Electrochemical Polymerization of Biotinyl-Tyramide', Electroanalysis, vol. 10, no. 1, pp. 58-60.
Yang ST, Witkowski A, Hutchins RS, Scott DL, Bachas LG. Biotin-Modified Surfaces by Electrochemical Polymerization of Biotinyl-Tyramide. Electroanalysis. 1998 Jan;10(1):58-60.
Yang, Seung T. ; Witkowski, Allan ; Hutchins, Richard S. ; Scott, Donna L. ; Bachas, Leonidas G. / Biotin-Modified Surfaces by Electrochemical Polymerization of Biotinyl-Tyramide. In: Electroanalysis. 1998 ; Vol. 10, No. 1. pp. 58-60.
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