Abstract
Poly(biotinyl-tyramide) films were created by the electrochemical oxidation of biotinyl-tyramide. Electropolymerization in basic solutions such as NaOH/methanol or aqueous NaOH led to adherent poly(biotinyl) films on both glassy-carbon (GC) and indium(tin) oxide (ITO) electrodes. Cyclic voltammetry revealed that the resulting poly(biotinyl-tyramide) films passivated the electrode surface. Studies using an avidin-β-galactosidase conjugate showed that the films provide biotin sites capable of selectively binding avidin.
Original language | English (US) |
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Pages (from-to) | 58-60 |
Number of pages | 3 |
Journal | Electroanalysis |
Volume | 10 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1998 |
Externally published | Yes |
Keywords
- Biotinyl-tyramide
- Cyclic voltammetry
- Electrochemical polymerization
- Glassy carbon electrode
- Indium(tin)oxide electrode
- Selectively binding avidin
ASJC Scopus subject areas
- Analytical Chemistry
- Electrochemistry