Biosynthesis of thyrotropin-releasing hormone by a rat medullary thyroid carcinoma cell line

K. A. Sevarino, P. Wu, I. M D Jackson, B. A. Roos, G. Mandel, R. H. Goodman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Prepro-thyrotropin-releasing hormone (TRH) messenger RNA was detected in the rat medullary thyroid carcinoma cell line CA77. The RNA of 1.6 kilobases comigrated with that found in rat hypothalamus. Using three radioimmunoassays specific for pro-TRH-derived peptides, we demonstrated that CA77 cells synthesize high levels of immunoreactive TRH and all of the other pro-TRH-derived peptides identified in hypothalamic tissue. The relative levels of the pro-TRH-derived peptides also indicate that CA77 cells process the TRH precursor in a manner similar to hypothalamic tissue. CA77 cells provide a promising model system for further studies of prepro-TRH gene regulation and post-translational maturation.

Original languageEnglish
Pages (from-to)620-623
Number of pages4
JournalJournal of Biological Chemistry
Volume263
Issue number2
StatePublished - Jan 1 1988
Externally publishedYes

Fingerprint

Thyrotropin-Releasing Hormone
Biosynthesis
Rats
Cells
Cell Line
Peptides
Tissue
Gene expression
Hypothalamus
Radioimmunoassay
RNA
Messenger RNA
pro-thyrotropin releasing hormone
Medullary Thyroid cancer
Genes
prepro-thyrotropin-releasing hormone

ASJC Scopus subject areas

  • Biochemistry

Cite this

Sevarino, K. A., Wu, P., Jackson, I. M. D., Roos, B. A., Mandel, G., & Goodman, R. H. (1988). Biosynthesis of thyrotropin-releasing hormone by a rat medullary thyroid carcinoma cell line. Journal of Biological Chemistry, 263(2), 620-623.

Biosynthesis of thyrotropin-releasing hormone by a rat medullary thyroid carcinoma cell line. / Sevarino, K. A.; Wu, P.; Jackson, I. M D; Roos, B. A.; Mandel, G.; Goodman, R. H.

In: Journal of Biological Chemistry, Vol. 263, No. 2, 01.01.1988, p. 620-623.

Research output: Contribution to journalArticle

Sevarino, KA, Wu, P, Jackson, IMD, Roos, BA, Mandel, G & Goodman, RH 1988, 'Biosynthesis of thyrotropin-releasing hormone by a rat medullary thyroid carcinoma cell line', Journal of Biological Chemistry, vol. 263, no. 2, pp. 620-623.
Sevarino KA, Wu P, Jackson IMD, Roos BA, Mandel G, Goodman RH. Biosynthesis of thyrotropin-releasing hormone by a rat medullary thyroid carcinoma cell line. Journal of Biological Chemistry. 1988 Jan 1;263(2):620-623.
Sevarino, K. A. ; Wu, P. ; Jackson, I. M D ; Roos, B. A. ; Mandel, G. ; Goodman, R. H. / Biosynthesis of thyrotropin-releasing hormone by a rat medullary thyroid carcinoma cell line. In: Journal of Biological Chemistry. 1988 ; Vol. 263, No. 2. pp. 620-623.
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