Biosynthesis of calcitonin by a rat medullary thyroid carcinoma cell line

R. S. Birnbaum, W. C. Mahoney, B. A. Roos

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The 32-amino acid form of the peptide hormone calcitonin is the product of a series of post-translational processing steps of a 13,400-dalton precursor, procalcitonin. We have now identified the steps involved in proteolytic paring of the precursor to the mature secretory form. Cultures of the CA-77 cell line were radiolabeled and the various forms of calcitonin were isolated by specific immunoprecipitation followed by fractionation on gel filtration and reversed-phase high performance liquid chromatography. Pulse-chase kinetics showed that procalcitonin was cleaved to a 6,500-dalton biosynthetic intermediate which was subsequently processed to the size of mature calcitonin (3,400 daltons). Partial microsequencing of the [35S] methionine-labeled intermediate indicated that the sequence consisted of the COOH-terminal 52 residues of procalcitonin. Partial microsequencing of the [35S]methionine- or [3H]proline-labeled 3,400-dalton species revealed that it was indistinguishable from naturally occurring, amidated calcitonin. These data define the major pathway for calcitonin biosynthesis in this neoplastic cell line and presumably in normal cells.

Original languageEnglish
Pages (from-to)699-703
Number of pages5
JournalJournal of Biological Chemistry
Volume261
Issue number2
StatePublished - Jan 1 1986

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Biosynthesis
Calcitonin
Rats
Cells
Cell Line
Methionine
Medullary Thyroid cancer
Peptide Hormones
High performance liquid chromatography
Reverse-Phase Chromatography
Fractionation
Cell culture
Immunoprecipitation
Proline
Gel Chromatography
Gels
High Pressure Liquid Chromatography
Amino Acids
Kinetics
Processing

ASJC Scopus subject areas

  • Biochemistry

Cite this

Birnbaum, R. S., Mahoney, W. C., & Roos, B. A. (1986). Biosynthesis of calcitonin by a rat medullary thyroid carcinoma cell line. Journal of Biological Chemistry, 261(2), 699-703.

Biosynthesis of calcitonin by a rat medullary thyroid carcinoma cell line. / Birnbaum, R. S.; Mahoney, W. C.; Roos, B. A.

In: Journal of Biological Chemistry, Vol. 261, No. 2, 01.01.1986, p. 699-703.

Research output: Contribution to journalArticle

Birnbaum, RS, Mahoney, WC & Roos, BA 1986, 'Biosynthesis of calcitonin by a rat medullary thyroid carcinoma cell line', Journal of Biological Chemistry, vol. 261, no. 2, pp. 699-703.
Birnbaum, R. S. ; Mahoney, W. C. ; Roos, B. A. / Biosynthesis of calcitonin by a rat medullary thyroid carcinoma cell line. In: Journal of Biological Chemistry. 1986 ; Vol. 261, No. 2. pp. 699-703.
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