Biosensor incorporating cell barrier architectures on ion selective electrodes for early screening of cancer

Gargi Ghosh, Leonidas G Bachas, Kimberly W. Anderson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Angiogenesis occurs during the early phase of cancer. Recruitment of new blood vessels by existing cancer cells leads to the release of higher concentrations of cytokines as compared to cells in healthy individuals. Some of the common cytokines observed at higher concentrations, such as vascular endothelial growth factor, basic fibroblast growth factor, hepatocyte growth factor and tumor necrosis factor-alpha, are also known to induce increased permeability across an endothelial cell monolayer. A whole-cell-based biosensor has been developed that can detect the presence of small quantities of the abovementioned cytokines individually and in different combinations. It was observed that the biosensor could differentiate between the cytokine concentrations observed in the sera of healthy individuals and cancer patients. The biosensor was also evaluated by exposing it to actual serum. These results demonstrated that the sensor can distinguish between healthy individuals and cancer patients and that the corresponding biosensor responses correlate with the stages of cancer.

Original languageEnglish (US)
Pages (from-to)2783-2791
Number of pages9
JournalAnalytical and Bioanalytical Chemistry
Volume391
Issue number8
DOIs
StatePublished - Aug 2008
Externally publishedYes

Fingerprint

Ion-Selective Electrodes
Ion selective electrodes
Biosensing Techniques
Early Detection of Cancer
Biosensors
Screening
Cytokines
Neoplasms
Hepatocyte Growth Factor
Endothelial cells
Blood vessels
Fibroblast Growth Factor 2
Vascular Endothelial Growth Factor A
Monolayers
Serum
Tumor Necrosis Factor-alpha
Cells
Blood Vessels
Permeability
Endothelial Cells

Keywords

  • Angiogenesis
  • Cell-based biosensor
  • Cytokines
  • Ion-selective electrode
  • Screening cancer

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Analytical Chemistry

Cite this

Biosensor incorporating cell barrier architectures on ion selective electrodes for early screening of cancer. / Ghosh, Gargi; Bachas, Leonidas G; Anderson, Kimberly W.

In: Analytical and Bioanalytical Chemistry, Vol. 391, No. 8, 08.2008, p. 2783-2791.

Research output: Contribution to journalArticle

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