Abstract
This work reports a bi-enzymatic biosensor for monitoring uric acid (UA) in biofluids extracted from wound, perilesional skin and healthy skin. The biosensor is composed of uricase (UOx) as a biocatalyst for UA oxidation, horseradish peroxidase (HRP) for electron transfer and a nanocomposite of multi-walled carbon nanotubes (MWCNTs) and Au nanoparticles (AuNPs) as the substrate. The bi-enzymatic approach provided a two-fold enhancement in current response, while the nanocomposite facilitated higher enzyme loading and fast electron transfer enabling a 2-fold increase in current response. The biosensor was able to measure UA levels in human wound exudate and in biofluids extracted from perilesional and healthy skin. The biosensor exhibited a lowest detectable concentration of 9.91 μM with a sensitivity of 2.5 nA μM−1. The UA levels in wound exudate and biofluid extract from perilesional skin were 3.7 and 1.2 times higher than in healthy skin. This result opened a potential way to use the biofluid in wound proximity as a biomarker for prolonged measurements, preventing electrode occlusion and sensor fouling by the wound debris. The electrode fouling studies demonstrated that the electrode with adsorbed wound exudate had 1.5 times reduction in current compared to electrode adsorbed with biofluid extract from perilesional skin.
| Original language | English (US) |
|---|---|
| Pages (from-to) | B830-B836 |
| Journal | Journal of the Electrochemical Society |
| Volume | 166 |
| Issue number | 10 |
| DOIs | |
| State | Published - Jan 1 2019 |
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ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Renewable Energy, Sustainability and the Environment
- Surfaces, Coatings and Films
- Electrochemistry
- Materials Chemistry
Cite this
Biosensor for monitoring uric acid in wound and its proximity : A potential wound diagnostic tool. / Bhushan, Pulak; Umasankar, Yogeswaran; RoyChoudhury, Sohini; Hirt, Penelope A.; MacQuhaec, Flor E.; Borda, Luis J.; Lev-Tov, Hadar A.; Kirsner, Robert S.; Bhansali, Shekhar.
In: Journal of the Electrochemical Society, Vol. 166, No. 10, 01.01.2019, p. B830-B836.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biosensor for monitoring uric acid in wound and its proximity
T2 - A potential wound diagnostic tool
AU - Bhushan, Pulak
AU - Umasankar, Yogeswaran
AU - RoyChoudhury, Sohini
AU - Hirt, Penelope A.
AU - MacQuhaec, Flor E.
AU - Borda, Luis J.
AU - Lev-Tov, Hadar A.
AU - Kirsner, Robert S.
AU - Bhansali, Shekhar
PY - 2019/1/1
Y1 - 2019/1/1
N2 - This work reports a bi-enzymatic biosensor for monitoring uric acid (UA) in biofluids extracted from wound, perilesional skin and healthy skin. The biosensor is composed of uricase (UOx) as a biocatalyst for UA oxidation, horseradish peroxidase (HRP) for electron transfer and a nanocomposite of multi-walled carbon nanotubes (MWCNTs) and Au nanoparticles (AuNPs) as the substrate. The bi-enzymatic approach provided a two-fold enhancement in current response, while the nanocomposite facilitated higher enzyme loading and fast electron transfer enabling a 2-fold increase in current response. The biosensor was able to measure UA levels in human wound exudate and in biofluids extracted from perilesional and healthy skin. The biosensor exhibited a lowest detectable concentration of 9.91 μM with a sensitivity of 2.5 nA μM−1. The UA levels in wound exudate and biofluid extract from perilesional skin were 3.7 and 1.2 times higher than in healthy skin. This result opened a potential way to use the biofluid in wound proximity as a biomarker for prolonged measurements, preventing electrode occlusion and sensor fouling by the wound debris. The electrode fouling studies demonstrated that the electrode with adsorbed wound exudate had 1.5 times reduction in current compared to electrode adsorbed with biofluid extract from perilesional skin.
AB - This work reports a bi-enzymatic biosensor for monitoring uric acid (UA) in biofluids extracted from wound, perilesional skin and healthy skin. The biosensor is composed of uricase (UOx) as a biocatalyst for UA oxidation, horseradish peroxidase (HRP) for electron transfer and a nanocomposite of multi-walled carbon nanotubes (MWCNTs) and Au nanoparticles (AuNPs) as the substrate. The bi-enzymatic approach provided a two-fold enhancement in current response, while the nanocomposite facilitated higher enzyme loading and fast electron transfer enabling a 2-fold increase in current response. The biosensor was able to measure UA levels in human wound exudate and in biofluids extracted from perilesional and healthy skin. The biosensor exhibited a lowest detectable concentration of 9.91 μM with a sensitivity of 2.5 nA μM−1. The UA levels in wound exudate and biofluid extract from perilesional skin were 3.7 and 1.2 times higher than in healthy skin. This result opened a potential way to use the biofluid in wound proximity as a biomarker for prolonged measurements, preventing electrode occlusion and sensor fouling by the wound debris. The electrode fouling studies demonstrated that the electrode with adsorbed wound exudate had 1.5 times reduction in current compared to electrode adsorbed with biofluid extract from perilesional skin.
UR - http://www.scopus.com/inward/record.url?scp=85072922486&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85072922486&partnerID=8YFLogxK
U2 - 10.1149/2.1441910jes
DO - 10.1149/2.1441910jes
M3 - Article
AN - SCOPUS:85072922486
VL - 166
SP - B830-B836
JO - Journal of the Electrochemical Society
JF - Journal of the Electrochemical Society
SN - 0013-4651
IS - 10
ER -