Biophysical characterization reveals structural disorder in the developmental transcriptional regulator LBH

Hassan Al-Ali, Megan E. Rieger, Kenneth L. Seldeen, Thomas K. Harris, Amjad Farooq, Karoline J. Briegel

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Limb-bud and heart (LBH) is a key transcriptional regulator in vertebrates with pivotal roles in embryonic development and human disease. Herein, using a diverse array of biophysical techniques, we report the first structural characterization of LBH pertinent to its biological function. Our data reveal that LBH is structurally disordered with no discernable secondary or tertiary structure and exudes rod-like properties in solution. Consistent with these observations, we also demonstrate that LBH is conformationally flexible and thus may be capable of adapting distinct conformations under specific physiological contexts. We propose that LBH is a member of the intrinsically disordered protein (IDP) family, and that conformational plasticity may play a significant role in modulating LBH-dependent transcriptional processes.

Original languageEnglish (US)
Pages (from-to)1104-1109
Number of pages6
JournalBiochemical and biophysical research communications
Volume391
Issue number1
DOIs
StatePublished - Jan 1 2010

Keywords

  • Embryonic development
  • Intrinsically disordered proteins
  • LBH
  • Transcriptional regulation
  • Vertebrates

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Biophysical characterization reveals structural disorder in the developmental transcriptional regulator LBH'. Together they form a unique fingerprint.

  • Cite this