Biophysical analysis of binding of WW domains of the YAP2 transcriptional regulator to PPXY motifs within WBP1 and WBP2 adaptors

Caleb B. McDonald, Samantha K N McIntosh, David C. Mikles, Vikas Bhat, Brian J. Deegan, Kenneth L. Seldeen, Ali M. Saeed, Laura Buffa, Marius Sudol, Zafar Nawaz, Amjad Farooq

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The YAP2 transcriptional regulator mediates a plethora of cellular functions, including the newly discovered Hippo tumor suppressor pathway, by virtue of its ability to recognize WBP1 and WBP2 signaling adaptors among a wide variety of other ligands. Herein, using isothermal titration calorimery and circular dichroism in combination with molecular modeling and molecular dynamics, we provide evidence that the WW1 and WW2 domains of YAP2 recognize various PPXY motifs within WBP1 and WBP2 in a highly promiscuous and subtle manner. Thus, although both WW domains strictly require the integrity of the consensus PPXY sequence, nonconsensus residues within and flanking this motif are not critical for high-affinity binding, implying that they most likely play a role in stabilizing the polyproline type II helical conformation of the PPXY ligands. Of particular interest is the observation that both WW domains bind to a PPXYXG motif with highest affinity, implicating a preference for a nonbulky and flexible glycine one residue to the C-terminal side of the consensus tyrosine. Importantly, a large set of residues within both WW domains and the PPXY motifs appear to undergo rapid fluctuations on a nanosecond time scale, suggesting that WW-ligand interactions are highly dynamic and that such conformational entropy may be an integral part of the reversible and temporal nature of cellular signaling cascades. Collectively, our study sheds light on the molecular determinants of a key WW-ligand interaction pertinent to cellular functions in health and disease.

Original languageEnglish
Pages (from-to)9616-9627
Number of pages12
JournalBiochemistry
Volume50
Issue number44
DOIs
StatePublished - Nov 8 2011

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Ligands
Cell signaling
Molecular modeling
Consensus Sequence
Dichroism
Entropy
Molecular Dynamics Simulation
Circular Dichroism
Titration
Glycine
Tyrosine
Conformations
Molecular dynamics
Tumors
Health
Neoplasms
PPXY ligand
polyproline

ASJC Scopus subject areas

  • Biochemistry

Cite this

Biophysical analysis of binding of WW domains of the YAP2 transcriptional regulator to PPXY motifs within WBP1 and WBP2 adaptors. / McDonald, Caleb B.; McIntosh, Samantha K N; Mikles, David C.; Bhat, Vikas; Deegan, Brian J.; Seldeen, Kenneth L.; Saeed, Ali M.; Buffa, Laura; Sudol, Marius; Nawaz, Zafar; Farooq, Amjad.

In: Biochemistry, Vol. 50, No. 44, 08.11.2011, p. 9616-9627.

Research output: Contribution to journalArticle

McDonald, CB, McIntosh, SKN, Mikles, DC, Bhat, V, Deegan, BJ, Seldeen, KL, Saeed, AM, Buffa, L, Sudol, M, Nawaz, Z & Farooq, A 2011, 'Biophysical analysis of binding of WW domains of the YAP2 transcriptional regulator to PPXY motifs within WBP1 and WBP2 adaptors', Biochemistry, vol. 50, no. 44, pp. 9616-9627. https://doi.org/10.1021/bi201286p
McDonald, Caleb B. ; McIntosh, Samantha K N ; Mikles, David C. ; Bhat, Vikas ; Deegan, Brian J. ; Seldeen, Kenneth L. ; Saeed, Ali M. ; Buffa, Laura ; Sudol, Marius ; Nawaz, Zafar ; Farooq, Amjad. / Biophysical analysis of binding of WW domains of the YAP2 transcriptional regulator to PPXY motifs within WBP1 and WBP2 adaptors. In: Biochemistry. 2011 ; Vol. 50, No. 44. pp. 9616-9627.
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