Bioorthogonal layer-by-layer encapsulation of pancreatic islets via hyperbranched polymers

Kerim M. Gattás-Asfura, Cherie L. Stabler

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Encapsulation of viable tissues via layer-by-layer polymer assembly provides a versatile platform for cell surface engineering, with nanoscale control over the capsule properties. Herein, we report the development of a hyperbranched polymer-based, ultrathin capsule architecture expressing bioorthogonal functionality and tailored physiochemical properties. Random carbodiimide-based condensation of 3,5-dicarboxyphenyl glycineamide on alginate yielded a highly branched polysaccharide with multiple, spatially restricted, and readily functionalizable terminal carboxylate moieties. Poly(ethylene glycol) (PEG) was utilized to link azido end groups to the structured alginate. Together with a phosphine-functionalized poly(amidoamine) dendrimer, nanoscale layer-by-layer coatings, covalently stabilized via Staudinger ligation, were assembled onto solid surfaces and pancreatic islets. The effects of electrostatic and/or bioorthogonal covalent interlayer interactions on the resulting coating efficiency and stability, as well as pancreatic islet viability and function, were studied. These hyperbranched polymers provide a flexible platform for the formation of covalently stabilized, ultrathin coatings on viable cells and tissues. In addition, the hyperbranched nature of the polymers presents a highly functionalized surface capable of bioorthogonal conjugation of additional bioactive or labeling motifs.

Original languageEnglish
Pages (from-to)9964-9974
Number of pages11
JournalACS Applied Materials and Interfaces
Volume5
Issue number20
DOIs
StatePublished - Nov 6 2013

Fingerprint

Islets of Langerhans
Encapsulation
Polymers
phosphine
Alginate
Coatings
Polyethylene glycols
Capsules
Tissue
Cell Engineering
Carbodiimides
Dendrimers
Ethylene Glycol
Polysaccharides
Static Electricity
Labeling
Ligation
Condensation
Electrostatics
alginic acid

Keywords

  • cell encapsulation
  • conformal coating
  • dendritic polymer
  • layer-by-layer
  • Staudinger ligation

ASJC Scopus subject areas

  • Materials Science(all)
  • Medicine(all)

Cite this

Bioorthogonal layer-by-layer encapsulation of pancreatic islets via hyperbranched polymers. / Gattás-Asfura, Kerim M.; Stabler, Cherie L.

In: ACS Applied Materials and Interfaces, Vol. 5, No. 20, 06.11.2013, p. 9964-9974.

Research output: Contribution to journalArticle

Gattás-Asfura, Kerim M. ; Stabler, Cherie L. / Bioorthogonal layer-by-layer encapsulation of pancreatic islets via hyperbranched polymers. In: ACS Applied Materials and Interfaces. 2013 ; Vol. 5, No. 20. pp. 9964-9974.
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