Abstract
Encapsulation of viable tissues via layer-by-layer polymer assembly provides a versatile platform for cell surface engineering, with nanoscale control over the capsule properties. Herein, we report the development of a hyperbranched polymer-based, ultrathin capsule architecture expressing bioorthogonal functionality and tailored physiochemical properties. Random carbodiimide-based condensation of 3,5-dicarboxyphenyl glycineamide on alginate yielded a highly branched polysaccharide with multiple, spatially restricted, and readily functionalizable terminal carboxylate moieties. Poly(ethylene glycol) (PEG) was utilized to link azido end groups to the structured alginate. Together with a phosphine-functionalized poly(amidoamine) dendrimer, nanoscale layer-by-layer coatings, covalently stabilized via Staudinger ligation, were assembled onto solid surfaces and pancreatic islets. The effects of electrostatic and/or bioorthogonal covalent interlayer interactions on the resulting coating efficiency and stability, as well as pancreatic islet viability and function, were studied. These hyperbranched polymers provide a flexible platform for the formation of covalently stabilized, ultrathin coatings on viable cells and tissues. In addition, the hyperbranched nature of the polymers presents a highly functionalized surface capable of bioorthogonal conjugation of additional bioactive or labeling motifs.
| Original language | English |
|---|---|
| Pages (from-to) | 9964-9974 |
| Number of pages | 11 |
| Journal | ACS Applied Materials and Interfaces |
| Volume | 5 |
| Issue number | 20 |
| DOIs | |
| State | Published - Nov 6 2013 |
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Keywords
- cell encapsulation
- conformal coating
- dendritic polymer
- layer-by-layer
- Staudinger ligation
ASJC Scopus subject areas
- Materials Science(all)
- Medicine(all)
Cite this
Bioorthogonal layer-by-layer encapsulation of pancreatic islets via hyperbranched polymers. / Gattás-Asfura, Kerim M.; Stabler, Cherie L.
In: ACS Applied Materials and Interfaces, Vol. 5, No. 20, 06.11.2013, p. 9964-9974.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bioorthogonal layer-by-layer encapsulation of pancreatic islets via hyperbranched polymers
AU - Gattás-Asfura, Kerim M.
AU - Stabler, Cherie L.
PY - 2013/11/6
Y1 - 2013/11/6
N2 - Encapsulation of viable tissues via layer-by-layer polymer assembly provides a versatile platform for cell surface engineering, with nanoscale control over the capsule properties. Herein, we report the development of a hyperbranched polymer-based, ultrathin capsule architecture expressing bioorthogonal functionality and tailored physiochemical properties. Random carbodiimide-based condensation of 3,5-dicarboxyphenyl glycineamide on alginate yielded a highly branched polysaccharide with multiple, spatially restricted, and readily functionalizable terminal carboxylate moieties. Poly(ethylene glycol) (PEG) was utilized to link azido end groups to the structured alginate. Together with a phosphine-functionalized poly(amidoamine) dendrimer, nanoscale layer-by-layer coatings, covalently stabilized via Staudinger ligation, were assembled onto solid surfaces and pancreatic islets. The effects of electrostatic and/or bioorthogonal covalent interlayer interactions on the resulting coating efficiency and stability, as well as pancreatic islet viability and function, were studied. These hyperbranched polymers provide a flexible platform for the formation of covalently stabilized, ultrathin coatings on viable cells and tissues. In addition, the hyperbranched nature of the polymers presents a highly functionalized surface capable of bioorthogonal conjugation of additional bioactive or labeling motifs.
AB - Encapsulation of viable tissues via layer-by-layer polymer assembly provides a versatile platform for cell surface engineering, with nanoscale control over the capsule properties. Herein, we report the development of a hyperbranched polymer-based, ultrathin capsule architecture expressing bioorthogonal functionality and tailored physiochemical properties. Random carbodiimide-based condensation of 3,5-dicarboxyphenyl glycineamide on alginate yielded a highly branched polysaccharide with multiple, spatially restricted, and readily functionalizable terminal carboxylate moieties. Poly(ethylene glycol) (PEG) was utilized to link azido end groups to the structured alginate. Together with a phosphine-functionalized poly(amidoamine) dendrimer, nanoscale layer-by-layer coatings, covalently stabilized via Staudinger ligation, were assembled onto solid surfaces and pancreatic islets. The effects of electrostatic and/or bioorthogonal covalent interlayer interactions on the resulting coating efficiency and stability, as well as pancreatic islet viability and function, were studied. These hyperbranched polymers provide a flexible platform for the formation of covalently stabilized, ultrathin coatings on viable cells and tissues. In addition, the hyperbranched nature of the polymers presents a highly functionalized surface capable of bioorthogonal conjugation of additional bioactive or labeling motifs.
KW - cell encapsulation
KW - conformal coating
KW - dendritic polymer
KW - layer-by-layer
KW - Staudinger ligation
UR - http://www.scopus.com/inward/record.url?scp=84886776007&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84886776007&partnerID=8YFLogxK
U2 - 10.1021/am401981g
DO - 10.1021/am401981g
M3 - Article
C2 - 24063764
AN - SCOPUS:84886776007
VL - 5
SP - 9964
EP - 9974
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
SN - 1944-8244
IS - 20
ER -