Biomimetic chemical sensors using nanoelectronic readout of olfactory receptor proteins

Brett R. Goldsmith, Joseph J. Mitala, Jesusa Josue, Ana Castro, Mitchell B. Lerner, Timothy H. Bayburt, Samuel M. Khamis, Ryan A. Jones, Joseph G. Brand, Stephen G. Sligar, Charles W Luetje, Alan Gelperin, Paul A. Rhodes, Bohdana M. Discher, A. T Charlie Johnson

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

We have designed and implemented a practical nanoelectronic interface to G-protein coupled receptors (GPCRs), a large family of membrane proteins whose roles in the detection of molecules outside eukaryotic cells make them important pharmaceutical targets. Specifically, we have coupled olfactory receptor proteins (ORs) with carbon nanotube transistors. The resulting devices transduce signals associated with odorant binding to ORs in the gas phase under ambient conditions and show responses that are in excellent agreement with results from established assays for OR-ligand binding. The work represents significant progress on a path toward a bioelectronic nose that can be directly compared to biological olfactory systems as well as a general method for the study of GPCR function in multiple domains using electronic readout.

Original languageEnglish
Pages (from-to)5408-5416
Number of pages9
JournalACS Nano
Volume5
Issue number7
DOIs
StatePublished - Jul 26 2011

Fingerprint

Odorant Receptors
Nanoelectronics
biomimetics
Biomimetics
G-Protein-Coupled Receptors
Chemical sensors
readout
proteins
Proteins
Carbon Nanotubes
sensors
Biological systems
Assays
Membrane Proteins
Transistors
Gases
Ligands
Molecules
Pharmaceutical Preparations
Drug products

Keywords

  • bioelectronics
  • field effect transistor
  • nanotube
  • olfactory receptor proteins
  • vapor sensor

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Goldsmith, B. R., Mitala, J. J., Josue, J., Castro, A., Lerner, M. B., Bayburt, T. H., ... Johnson, A. T. C. (2011). Biomimetic chemical sensors using nanoelectronic readout of olfactory receptor proteins. ACS Nano, 5(7), 5408-5416. https://doi.org/10.1021/nn200489j

Biomimetic chemical sensors using nanoelectronic readout of olfactory receptor proteins. / Goldsmith, Brett R.; Mitala, Joseph J.; Josue, Jesusa; Castro, Ana; Lerner, Mitchell B.; Bayburt, Timothy H.; Khamis, Samuel M.; Jones, Ryan A.; Brand, Joseph G.; Sligar, Stephen G.; Luetje, Charles W; Gelperin, Alan; Rhodes, Paul A.; Discher, Bohdana M.; Johnson, A. T Charlie.

In: ACS Nano, Vol. 5, No. 7, 26.07.2011, p. 5408-5416.

Research output: Contribution to journalArticle

Goldsmith, BR, Mitala, JJ, Josue, J, Castro, A, Lerner, MB, Bayburt, TH, Khamis, SM, Jones, RA, Brand, JG, Sligar, SG, Luetje, CW, Gelperin, A, Rhodes, PA, Discher, BM & Johnson, ATC 2011, 'Biomimetic chemical sensors using nanoelectronic readout of olfactory receptor proteins', ACS Nano, vol. 5, no. 7, pp. 5408-5416. https://doi.org/10.1021/nn200489j
Goldsmith BR, Mitala JJ, Josue J, Castro A, Lerner MB, Bayburt TH et al. Biomimetic chemical sensors using nanoelectronic readout of olfactory receptor proteins. ACS Nano. 2011 Jul 26;5(7):5408-5416. https://doi.org/10.1021/nn200489j
Goldsmith, Brett R. ; Mitala, Joseph J. ; Josue, Jesusa ; Castro, Ana ; Lerner, Mitchell B. ; Bayburt, Timothy H. ; Khamis, Samuel M. ; Jones, Ryan A. ; Brand, Joseph G. ; Sligar, Stephen G. ; Luetje, Charles W ; Gelperin, Alan ; Rhodes, Paul A. ; Discher, Bohdana M. ; Johnson, A. T Charlie. / Biomimetic chemical sensors using nanoelectronic readout of olfactory receptor proteins. In: ACS Nano. 2011 ; Vol. 5, No. 7. pp. 5408-5416.
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