Abstract
The development of a bioluminescent immunosensor is reported for the determination of zearalenone (ZEA) based on a peptide mimetic identified by phage display. The peptide mimetic GW, with a peptide sequence GWWGPYGEIELL, was used to create recombinant fusion proteins with the bioluminescent Gaussia luciferase (GLuc) that were directly used as tracers for toxin detection in a competitive immunoassay without the need for secondary antibodies or further labeling. The bioluminescent sensor, based on protein G–coupled magnetic beads for antibody immobilization, enabled determination of ZEA with a detection limit of 4.2 ng mL−1 (corresponding to 420 μg kg−1 in food samples) and an IC50 value of 11.0 ng mL−1. The sensor performance was evaluated in spiked maize and wheat samples, with recoveries ranging from 87 to 106% (RSD < 20%, n = 3). Finally, the developed method was applied to the analysis of a naturally contaminated reference matrix material and good agreement with the reported concentrations was obtained. Graphical abstract[Figure not available: see fulltext.]
Original language | English (US) |
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Article number | 547 |
Journal | Mikrochimica Acta |
Volume | 187 |
Issue number | 10 |
DOIs | |
State | Published - Oct 1 2020 |
Keywords
- Bioluminescence
- Food safety
- Gaussia luciferase
- Mycotoxin
- Zearalenone
ASJC Scopus subject areas
- Analytical Chemistry