Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein

Riikka Peltomaa; Sabrina Fikacek; Elena Benito-Peña; Rodrigo Barderas; Trajen Head; Sapna Deo; Sylvia Daunert; María C. Moreno-Bondi

doi:10.1007/s00604-020-04538-7

Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein

Riikka Peltomaa, Sabrina Fikacek, Elena Benito-Peña, Rodrigo Barderas, Trajen Head, Sapna Deo, Sylvia Daunert, María C. Moreno-Bondi

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The development of a bioluminescent immunosensor is reported for the determination of zearalenone (ZEA) based on a peptide mimetic identified by phage display. The peptide mimetic GW, with a peptide sequence GWWGPYGEIELL, was used to create recombinant fusion proteins with the bioluminescent Gaussia luciferase (GLuc) that were directly used as tracers for toxin detection in a competitive immunoassay without the need for secondary antibodies or further labeling. The bioluminescent sensor, based on protein G–coupled magnetic beads for antibody immobilization, enabled determination of ZEA with a detection limit of 4.2 ng mL⁻¹ (corresponding to 420 μg kg⁻¹ in food samples) and an IC₅₀ value of 11.0 ng mL⁻¹. The sensor performance was evaluated in spiked maize and wheat samples, with recoveries ranging from 87 to 106% (RSD < 20%, n = 3). Finally, the developed method was applied to the analysis of a naturally contaminated reference matrix material and good agreement with the reported concentrations was obtained. Graphical abstract[Figure not available: see fulltext.]

Original language	English (US)
Article number	547
Journal	Mikrochimica Acta
Volume	187
Issue number	10
DOIs	https://doi.org/10.1007/s00604-020-04538-7
State	Published - Oct 1 2020

Keywords

Bioluminescence
Food safety
Gaussia luciferase
Mycotoxin
Zearalenone

ASJC Scopus subject areas

Analytical Chemistry

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Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein. / Peltomaa, Riikka; Fikacek, Sabrina; Benito-Peña, Elena; Barderas, Rodrigo; Head, Trajen; Deo, Sapna ; Daunert, Sylvia; Moreno-Bondi, María C.

In: Mikrochimica Acta, Vol. 187, No. 10, 547, 01.10.2020.

Research output: Contribution to journal › Article › peer-review

@article{61b3daa95cad4274940fb4f82cc79bfd,

title = "Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein",

abstract = "The development of a bioluminescent immunosensor is reported for the determination of zearalenone (ZEA) based on a peptide mimetic identified by phage display. The peptide mimetic GW, with a peptide sequence GWWGPYGEIELL, was used to create recombinant fusion proteins with the bioluminescent Gaussia luciferase (GLuc) that were directly used as tracers for toxin detection in a competitive immunoassay without the need for secondary antibodies or further labeling. The bioluminescent sensor, based on protein G–coupled magnetic beads for antibody immobilization, enabled determination of ZEA with a detection limit of 4.2 ng mL−1 (corresponding to 420 μg kg−1 in food samples) and an IC50 value of 11.0 ng mL−1. The sensor performance was evaluated in spiked maize and wheat samples, with recoveries ranging from 87 to 106% (RSD < 20%, n = 3). Finally, the developed method was applied to the analysis of a naturally contaminated reference matrix material and good agreement with the reported concentrations was obtained. Graphical abstract[Figure not available: see fulltext.]",

keywords = "Bioluminescence, Food safety, Gaussia luciferase, Mycotoxin, Zearalenone",

author = "Riikka Peltomaa and Sabrina Fikacek and Elena Benito-Pe{\~n}a and Rodrigo Barderas and Trajen Head and Sapna Deo and Sylvia Daunert and Moreno-Bondi, {Mar{\'i}a C.}",

note = "Funding Information: This study was supported by the Spanish Ministry of Science, Innovation and Universities (RTI2018–096410-B-C21). R.P. acknowledges UCM for a research grant. S. Daunert and S. Deo would like to thank the National Institutes of Health, NIGMS (R01GM114321, R01GM127706). This work was also supported by the National Science Foundation grant (CHE-1506740, CBET-1841419) to S. Daunert and S. Deo. S. Daunert is grateful to the Miller School of Medicine of the University of Miami for the Lucille P. Markey Chair in Biochemistry and Molecular Biology. Acknowledgments Funding Information: Belen Pati?o from the Department of Biology (Universidad Complutense de Madrid) is gratefully acknowledged for providing the blank maize samples analyzed in this work. Publisher Copyright: {\textcopyright} 2020, Springer-Verlag GmbH Austria, part of Springer Nature. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

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doi = "10.1007/s00604-020-04538-7",

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T1 - Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein

AU - Peltomaa, Riikka

AU - Fikacek, Sabrina

AU - Benito-Peña, Elena

AU - Barderas, Rodrigo

AU - Head, Trajen

AU - Deo, Sapna

AU - Daunert, Sylvia

AU - Moreno-Bondi, María C.

N1 - Funding Information: This study was supported by the Spanish Ministry of Science, Innovation and Universities (RTI2018–096410-B-C21). R.P. acknowledges UCM for a research grant. S. Daunert and S. Deo would like to thank the National Institutes of Health, NIGMS (R01GM114321, R01GM127706). This work was also supported by the National Science Foundation grant (CHE-1506740, CBET-1841419) to S. Daunert and S. Deo. S. Daunert is grateful to the Miller School of Medicine of the University of Miami for the Lucille P. Markey Chair in Biochemistry and Molecular Biology. Acknowledgments Funding Information: Belen Pati?o from the Department of Biology (Universidad Complutense de Madrid) is gratefully acknowledged for providing the blank maize samples analyzed in this work. Publisher Copyright: © 2020, Springer-Verlag GmbH Austria, part of Springer Nature. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/10/1

Y1 - 2020/10/1

N2 - The development of a bioluminescent immunosensor is reported for the determination of zearalenone (ZEA) based on a peptide mimetic identified by phage display. The peptide mimetic GW, with a peptide sequence GWWGPYGEIELL, was used to create recombinant fusion proteins with the bioluminescent Gaussia luciferase (GLuc) that were directly used as tracers for toxin detection in a competitive immunoassay without the need for secondary antibodies or further labeling. The bioluminescent sensor, based on protein G–coupled magnetic beads for antibody immobilization, enabled determination of ZEA with a detection limit of 4.2 ng mL−1 (corresponding to 420 μg kg−1 in food samples) and an IC50 value of 11.0 ng mL−1. The sensor performance was evaluated in spiked maize and wheat samples, with recoveries ranging from 87 to 106% (RSD < 20%, n = 3). Finally, the developed method was applied to the analysis of a naturally contaminated reference matrix material and good agreement with the reported concentrations was obtained. Graphical abstract[Figure not available: see fulltext.]

AB - The development of a bioluminescent immunosensor is reported for the determination of zearalenone (ZEA) based on a peptide mimetic identified by phage display. The peptide mimetic GW, with a peptide sequence GWWGPYGEIELL, was used to create recombinant fusion proteins with the bioluminescent Gaussia luciferase (GLuc) that were directly used as tracers for toxin detection in a competitive immunoassay without the need for secondary antibodies or further labeling. The bioluminescent sensor, based on protein G–coupled magnetic beads for antibody immobilization, enabled determination of ZEA with a detection limit of 4.2 ng mL−1 (corresponding to 420 μg kg−1 in food samples) and an IC50 value of 11.0 ng mL−1. The sensor performance was evaluated in spiked maize and wheat samples, with recoveries ranging from 87 to 106% (RSD < 20%, n = 3). Finally, the developed method was applied to the analysis of a naturally contaminated reference matrix material and good agreement with the reported concentrations was obtained. Graphical abstract[Figure not available: see fulltext.]

KW - Bioluminescence

KW - Food safety

KW - Gaussia luciferase

KW - Mycotoxin

KW - Zearalenone

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AN - SCOPUS:85090275349

VL - 187

JO - Mikrochimica Acta

JF - Mikrochimica Acta

SN - 0026-3672

IS - 10

M1 - 547

ER -

Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein

Abstract

Keywords

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