Bioluminescent and micro-computed tomography imaging of bone repair induced by fibrin-binding growth factors

Olaia F. Vila, Mikaël M. Martino, Laura Nebuloni, Gisela Kuhn, Soledad Pérez-Amodio, Ralph Müller, Jeffrey A. Hubbell, Nuria Rubio, Jerónimo Blanco

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

In this work we have evaluated the capacity of bone morphogenetic protein-2 (BMP-2) and fibrin-binding platelet-derived growth factor-BB (PDGF-BB) to support cell growth and induce bone regeneration using two different imaging technologies to improve the understanding of structural and organizational processes participating in tissue repair. Human mesenchymal stem cells from adipose tissue (hAMSCs) expressing two luciferase genes, one under the control of the cytomegalovirus (CMV) promoter and the other under the control of a tissue-specific promoter (osteocalcin or platelet endothelial cell adhesion molecule), were seeded in fibrin matrices containing BMP-2 and fibrin-binding PDGF-BB, and further implanted intramuscularly or in a mouse calvarial defect. Then, cell growth and bone regeneration were monitored by bioluminescence imaging (BLI) to analyze the evolution of target gene expression, indicative of cell differentiation towards the osteoblastic and endothelial lineages. Non-invasive imaging was supplemented with micro-computed tomography (μCT) to evaluate bone regeneration and high-resolution μCT of vascular casts. Results from BLI showed hAMSC growth during the first week in all cases, followed by a rapid decrease in cell number; as well as an increment of osteocalcin but not PECAM-1 expression 3 weeks after implantation. Results from μCT show that the delivery of BMP-2 and PDGF-BB by fibrin induced the formation of more bone and improves vascularization, resulting in more abundant and thicker vessels, in comparison with controls. Although the inclusion of hAMSCs in the fibrin matrices made no significant difference in any of these parameters, there was a significant increment in the connectivity of the vascular network in defects treated with hAMSCs.

Original languageEnglish (US)
Pages (from-to)4377-4389
Number of pages13
JournalActa Biomaterialia
Volume10
Issue number10
DOIs
StatePublished - Oct 1 2014

Keywords

  • Angiogenesis
  • Bioluminescence imaging
  • Bone regeneration
  • Fibrin
  • Mesenchymal stem cell

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

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    Vila, O. F., Martino, M. M., Nebuloni, L., Kuhn, G., Pérez-Amodio, S., Müller, R., Hubbell, J. A., Rubio, N., & Blanco, J. (2014). Bioluminescent and micro-computed tomography imaging of bone repair induced by fibrin-binding growth factors. Acta Biomaterialia, 10(10), 4377-4389. https://doi.org/10.1016/j.actbio.2014.05.028