Bioluminescence imaging of calvarial bone repair using bone marrow and adipose tissue-derived mesenchymal stem cells

Irene R. Dégano, Marta Vilalta, Juli R. Bagó, Annette M. Matthies, Jeffrey A. Hubbell, Helen Dimitriou, Paolo Bianco, Nuria Rubio, Jerónimo Blanco

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

A combined strategy using bioluminescence imaging, bone densitometry and histology was used to analyze the bone regeneration capacity of human bone marrow (hBMSC) and adipose tissue (hAMSC) mesenchymal stem cells, seeded in an osteoconductive arginine-glycine-aspartate (RGD) crosslinked hydrogel scaffold, implanted in a mouse calvarial bone defect. We show that firefly luciferase labeled stem cells can be monitored in vivo through a prolonged 90 days period, during which hBMSCs survive better than hAMSCs and that the density of scaffold bearing defects increased significantly more than that of defects without scaffolds.

Original languageEnglish (US)
Pages (from-to)427-437
Number of pages11
JournalBiomaterials
Volume29
Issue number4
DOIs
StatePublished - Feb 1 2008

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Keywords

  • Cell differentiation
  • Enhanced green fluorescent protein (eGFP)
  • In vivo imaging
  • Luciferase
  • Scaffold

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Dégano, I. R., Vilalta, M., Bagó, J. R., Matthies, A. M., Hubbell, J. A., Dimitriou, H., Bianco, P., Rubio, N., & Blanco, J. (2008). Bioluminescence imaging of calvarial bone repair using bone marrow and adipose tissue-derived mesenchymal stem cells. Biomaterials, 29(4), 427-437. https://doi.org/10.1016/j.biomaterials.2007.10.006