Abstract
Electrospray ionization mass spectrometry has revolutionized the way lipids are studied. In this work, we present a tutorial for analyzing class-specific lipid spectra obtained from a triple quadrupole mass spectrometer. The open-source software MZmine 2.21 is used, coupled with LIPID MAPS databases. Here, we describe the steps for lipid identification, ratiometric quantification, and briefly address the differences to the analyses when using direct infusion versus tandem liquid chromatography–mass spectrometry (LC–MS). We also provide a tutorial and equations for quantification of lipid amounts using synthetic lipid standards and normalization to a protein amount.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 141-147 |
| Number of pages | 7 |
| Journal | Methods in Molecular Biology |
| Volume | 1609 |
| DOIs | |
| State | Published - 2017 |
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Keywords
- Bioinformatic analysis
- Lipid profile
- Lipidomic analysis
- MZmine 2.21
- Ratiometric quantification
- Standards
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Bioinformatics pertinent to lipid analysis in biological samples. / Ma, Justin; Arbelo, Ulises; Guerra, Yenifer; Aribindi, Katyayini; Bhattacharya, Sanjoy K; Pelaez, Daniel.
In: Methods in Molecular Biology, Vol. 1609, 2017, p. 141-147.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bioinformatics pertinent to lipid analysis in biological samples
AU - Ma, Justin
AU - Arbelo, Ulises
AU - Guerra, Yenifer
AU - Aribindi, Katyayini
AU - Bhattacharya, Sanjoy K
AU - Pelaez, Daniel
PY - 2017
Y1 - 2017
N2 - Electrospray ionization mass spectrometry has revolutionized the way lipids are studied. In this work, we present a tutorial for analyzing class-specific lipid spectra obtained from a triple quadrupole mass spectrometer. The open-source software MZmine 2.21 is used, coupled with LIPID MAPS databases. Here, we describe the steps for lipid identification, ratiometric quantification, and briefly address the differences to the analyses when using direct infusion versus tandem liquid chromatography–mass spectrometry (LC–MS). We also provide a tutorial and equations for quantification of lipid amounts using synthetic lipid standards and normalization to a protein amount.
AB - Electrospray ionization mass spectrometry has revolutionized the way lipids are studied. In this work, we present a tutorial for analyzing class-specific lipid spectra obtained from a triple quadrupole mass spectrometer. The open-source software MZmine 2.21 is used, coupled with LIPID MAPS databases. Here, we describe the steps for lipid identification, ratiometric quantification, and briefly address the differences to the analyses when using direct infusion versus tandem liquid chromatography–mass spectrometry (LC–MS). We also provide a tutorial and equations for quantification of lipid amounts using synthetic lipid standards and normalization to a protein amount.
KW - Bioinformatic analysis
KW - Lipid profile
KW - Lipidomic analysis
KW - MZmine 2.21
KW - Ratiometric quantification
KW - Standards
UR - http://www.scopus.com/inward/record.url?scp=85021730142&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85021730142&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-6996-8_13
DO - 10.1007/978-1-4939-6996-8_13
M3 - Article
C2 - 28660580
AN - SCOPUS:85021730142
VL - 1609
SP - 141
EP - 147
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -