Bioimaging and self-assembly of lysozyme fibrils utilizing CdSe/ZnS quantum dots

Charles H. Vannoy; Jianmin Xu; Roger M. Leblanc

doi:10.1021/jp907839j

Bioimaging and self-assembly of lysozyme fibrils utilizing CdSe/ZnS quantum dots

Charles H. Vannoy, Jianmin Xu, Roger M. Leblanc

Chemistry

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Advances in recent nanoscience technologies have generated a new compilation of fluorescent biolabels derived from semiconductor quantum dots (QDs). Due to their properties of being water-soluble and biocompatible, these nanometer-sized probes prove to be a beneficial tool for biolabeling and bioimaging. We report on the molecular characterization of engineered bioconjugates comprised of highly luminescent dihydrolipoic acid (DHLA) capped CdSe/ZnS (core/shell) QDs attached to the native protein hen egg white lysozyme (HEWL) and/or its fibrous analogue. This research focuses on the development of a specific fluorescent labeling system and exploration of fibril formation. We confirmed the bioconjugation and formation of the HEWL-QDs and HEWL-QDs fibril model systems by various analytical techniques including agarose gel electrophoresis (AGE), confocal microscopy, transmission electron microscopy (TEM), atomic force microscopy (AFM), and Uv-vis and fluorescence spectroscopies. These bioconjugates could prove to be a potentially useful application in nanobiotechnology as a bioimaging tool and as a reference for a controlled biological self-assembly process.

Original language	English (US)
Pages (from-to)	766-773
Number of pages	8
Journal	Journal of Physical Chemistry C
Volume	114
Issue number	2
DOIs	https://doi.org/10.1021/jp907839j
State	Published - Jan 21 2010

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Energy(all)
Physical and Theoretical Chemistry
Surfaces, Coatings and Films

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title = "Bioimaging and self-assembly of lysozyme fibrils utilizing CdSe/ZnS quantum dots",

abstract = "Advances in recent nanoscience technologies have generated a new compilation of fluorescent biolabels derived from semiconductor quantum dots (QDs). Due to their properties of being water-soluble and biocompatible, these nanometer-sized probes prove to be a beneficial tool for biolabeling and bioimaging. We report on the molecular characterization of engineered bioconjugates comprised of highly luminescent dihydrolipoic acid (DHLA) capped CdSe/ZnS (core/shell) QDs attached to the native protein hen egg white lysozyme (HEWL) and/or its fibrous analogue. This research focuses on the development of a specific fluorescent labeling system and exploration of fibril formation. We confirmed the bioconjugation and formation of the HEWL-QDs and HEWL-QDs fibril model systems by various analytical techniques including agarose gel electrophoresis (AGE), confocal microscopy, transmission electron microscopy (TEM), atomic force microscopy (AFM), and Uv-vis and fluorescence spectroscopies. These bioconjugates could prove to be a potentially useful application in nanobiotechnology as a bioimaging tool and as a reference for a controlled biological self-assembly process.",

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