Bioimaging and self-assembly of lysozyme fibrils utilizing CdSe/ZnS quantum dots

Charles H. Vannoy, Jianmin Xu, Roger Leblanc

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Advances in recent nanoscience technologies have generated a new compilation of fluorescent biolabels derived from semiconductor quantum dots (QDs). Due to their properties of being water-soluble and biocompatible, these nanometer-sized probes prove to be a beneficial tool for biolabeling and bioimaging. We report on the molecular characterization of engineered bioconjugates comprised of highly luminescent dihydrolipoic acid (DHLA) capped CdSe/ZnS (core/shell) QDs attached to the native protein hen egg white lysozyme (HEWL) and/or its fibrous analogue. This research focuses on the development of a specific fluorescent labeling system and exploration of fibril formation. We confirmed the bioconjugation and formation of the HEWL-QDs and HEWL-QDs fibril model systems by various analytical techniques including agarose gel electrophoresis (AGE), confocal microscopy, transmission electron microscopy (TEM), atomic force microscopy (AFM), and Uv-vis and fluorescence spectroscopies. These bioconjugates could prove to be a potentially useful application in nanobiotechnology as a bioimaging tool and as a reference for a controlled biological self-assembly process.

Original languageEnglish
Pages (from-to)766-773
Number of pages8
JournalJournal of Physical Chemistry C
Volume114
Issue number2
DOIs
StatePublished - Jan 21 2010

Fingerprint

lysozyme
Muramidase
Self assembly
Semiconductor quantum dots
self assembly
eggs
Enzymes
quantum dots
Nanobiotechnology
Nanoscience
Confocal microscopy
Fluorescence spectroscopy
electrophoresis
Electrophoresis
Sepharose
Labeling
marking
Atomic force microscopy
Gels
atomic force microscopy

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Energy(all)

Cite this

Bioimaging and self-assembly of lysozyme fibrils utilizing CdSe/ZnS quantum dots. / Vannoy, Charles H.; Xu, Jianmin; Leblanc, Roger.

In: Journal of Physical Chemistry C, Vol. 114, No. 2, 21.01.2010, p. 766-773.

Research output: Contribution to journalArticle

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