Bioimaging and self-assembly of lysozyme fibrils utilizing CdSe/ZnS quantum dots

Charles H. Vannoy, Jianmin Xu, Roger M. Leblanc

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Advances in recent nanoscience technologies have generated a new compilation of fluorescent biolabels derived from semiconductor quantum dots (QDs). Due to their properties of being water-soluble and biocompatible, these nanometer-sized probes prove to be a beneficial tool for biolabeling and bioimaging. We report on the molecular characterization of engineered bioconjugates comprised of highly luminescent dihydrolipoic acid (DHLA) capped CdSe/ZnS (core/shell) QDs attached to the native protein hen egg white lysozyme (HEWL) and/or its fibrous analogue. This research focuses on the development of a specific fluorescent labeling system and exploration of fibril formation. We confirmed the bioconjugation and formation of the HEWL-QDs and HEWL-QDs fibril model systems by various analytical techniques including agarose gel electrophoresis (AGE), confocal microscopy, transmission electron microscopy (TEM), atomic force microscopy (AFM), and Uv-vis and fluorescence spectroscopies. These bioconjugates could prove to be a potentially useful application in nanobiotechnology as a bioimaging tool and as a reference for a controlled biological self-assembly process.

Original languageEnglish (US)
Pages (from-to)766-773
Number of pages8
JournalJournal of Physical Chemistry C
Volume114
Issue number2
DOIs
StatePublished - Jan 21 2010

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Energy(all)
  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films

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