Biofunctional membranes: electron paramagnetic resonance studies of the active site structure of enzymes site-specifically immobilized onto polymeric supports through molecular recognition

D. Allan Butterfield, Ram Subramaniam, Dibakar Bhattacharyya, Shekhar Vishwanath, Wei Huang, Leonidas Bachas

Research output: Contribution to journalConference articlepeer-review

3 Scopus citations

Abstract

Results are presented of studies on spin-labeled enzymes immobilized onto polymeric supports using site-specific immobilization. A different way to use molecular recognition to immobilize subtilisin is to utilize molecular biology, and site-directed mutagenesis in particular. It is shown that utilizing the principles of molecular recognition, one can immobilize enzymes onto polymeric supports making the resulting biofunctional membrane more active than with random immobilization.

Original languageEnglish (US)
Pages (from-to)602-603
Number of pages2
JournalPolymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering
Volume76
StatePublished - Jan 1 1997
Externally publishedYes
EventProceedings of the 1997 Spring ACS Meeting - San Francisco, CA, USA
Duration: Apr 13 1997Apr 17 1997

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Polymers and Plastics

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