Biofilm and scleral buckle-associated infections: A mechanism for persistence

S. P. Holland, J. S. Pulido, Darlene Miller, B. Ellis, Eduardo C Alfonso, M. Scott, J. W. Costerton

Research output: Contribution to journalArticle

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Abstract

Scleral buckle infections tend to be persistent as well as resistant to antimicrobial treatment. Often, scleral buckle infections require removal of the buckling elements for resolution. To determine if bacteria are able to persist on scleral buckles by elaborating a glycocalyx matrix or biofilm that offers protection against host defenses and antimicrobial treatment, the authors cultured 28 scleral buckle elements removed for infection and extrusion. Bacteria were isolated from 18 elements (64%). The most frequently isolated bacteria were Staphylococcus epidermidis and other coagulase-negative staphylococci (8), Staphylococcus aureus (3), corynebacteria (3), Mycobacterium chelonei (3), and Proteus mirabilis (3). Eleven (65%) of 17 buckles evaluated with scanning electron microscopy demonstrated the presence of bacteria encased in biofilm. Biofilm was demonstrated on the surfaces and ends of solid silicon elements. In the silicon sponges, biofilm also extended into the matrix of the sponges. The authors believe that bacterial production of biofilm offers an explanation for the persistence of scleral buckle infections and their ability to withstand antimicrobial treatment.

Original languageEnglish
Pages (from-to)933-938
Number of pages6
JournalOphthalmology
Volume98
Issue number6
StatePublished - Jan 1 1991

Fingerprint

Biofilms
Bacteria
Infection
Porifera
Silicon
Mycobacterium chelonae
Glycocalyx
Proteus mirabilis
Corynebacterium
Staphylococcus epidermidis
Coagulase
Staphylococcus
Electron Scanning Microscopy
Staphylococcus aureus

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Holland, S. P., Pulido, J. S., Miller, D., Ellis, B., Alfonso, E. C., Scott, M., & Costerton, J. W. (1991). Biofilm and scleral buckle-associated infections: A mechanism for persistence. Ophthalmology, 98(6), 933-938.

Biofilm and scleral buckle-associated infections : A mechanism for persistence. / Holland, S. P.; Pulido, J. S.; Miller, Darlene; Ellis, B.; Alfonso, Eduardo C; Scott, M.; Costerton, J. W.

In: Ophthalmology, Vol. 98, No. 6, 01.01.1991, p. 933-938.

Research output: Contribution to journalArticle

Holland, SP, Pulido, JS, Miller, D, Ellis, B, Alfonso, EC, Scott, M & Costerton, JW 1991, 'Biofilm and scleral buckle-associated infections: A mechanism for persistence', Ophthalmology, vol. 98, no. 6, pp. 933-938.
Holland SP, Pulido JS, Miller D, Ellis B, Alfonso EC, Scott M et al. Biofilm and scleral buckle-associated infections: A mechanism for persistence. Ophthalmology. 1991 Jan 1;98(6):933-938.
Holland, S. P. ; Pulido, J. S. ; Miller, Darlene ; Ellis, B. ; Alfonso, Eduardo C ; Scott, M. ; Costerton, J. W. / Biofilm and scleral buckle-associated infections : A mechanism for persistence. In: Ophthalmology. 1991 ; Vol. 98, No. 6. pp. 933-938.
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