Biocompatibility of bone marrow-derived mesenchymal stem cells in the rat inner ear following trans-tympanic administration

Adrien A. Eshraghi, Emre Ocak, Angela Zhu, Jeenu Mittal, Camron Davies, David Shahal, Erdogan Bulut, Rahul Sinha, Viraj Shah, Mario M. Perdomo, Rahul Mittal

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Recent advancements in stem cell therapy have led to an increased interest within the auditory community in exploring the potential of mesenchymal stem cells (MSCs) in the treatment of inner ear disorders. However, the biocompatibility of MSCs with the inner ear, especially when delivered non-surgically and in the immunocompetent cochlea, is not completely understood. In this study, we determined the effect of intratympanic administration of rodent bone marrow MSCs (BM-MSCs) on the inner ear in an immunocompetent rat model. The administration of MSCs did not lead to the generation of any oxidative stress in the rat inner ear. There was no significant production of proinflammatory cytokines, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and IL-12, due to BM-MSCs administration into the rat cochlea. BM-MSCs do not activate caspase 3 pathway, which plays a central role in sensory cell damage. Additionally, transferase dUTP nick end labeling (TUNEL) staining determined that there was no significant cell death associated with the administration of BM-MSCs. The results of the present study suggest that trans-tympanic administration of BM-MSCs does not result in oxidative stress or inflammatory response in the immunocompetent rat cochlea.

Original languageEnglish (US)
Article number1711
JournalJournal of Clinical Medicine
Volume9
Issue number6
DOIs
StatePublished - Jun 2020

Keywords

  • Caspase 3
  • Inner ear
  • Mesenchymal stem cells
  • Oxidative stress
  • Proinflammcytokines
  • TUNEL staining

ASJC Scopus subject areas

  • Medicine(all)

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