Biochemical studies of human syncytiotrophoblastic plasma membrane proteins

M. J. O'Sullivan, J. A. McIntyre, G. Warriner, W. Page Faulk

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Interest in the structure and function of the human syncytiotrophoblastic plasma membrane has been stimulated by the possibility that it may contribute to the acceptance of the foetal allograft. Preparations enriched in syncytiotrophoblastic plasma membrane microvilli have been isolated and their polypeptide composition analysed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. Extrinsic membrane proteins were identified by extraction with 3 M KCl and intrinsic membrane proteins were identified by solubilization of KCl-extracted membranes with the detergent sodium deoxycholate. A number of glycoproteins were isolated from detergent-solubilized syncytiotrophoblast membranes by absorption with wheat germ lectin-Sepharose followed by elution with N-acetyl-D-glucosamine. Since these glycoproteins are swept into the maternal blood during trophoblast deportation, methods for their isolation and characterization are necessary to describe more completely this aspect of materno-foetal immunobiology. The results presented here provide another step towards this goal.

Original languageEnglish
Pages (from-to)297-306
Number of pages10
JournalJournal of Reproductive Immunology
Volume3
Issue number5
DOIs
StatePublished - Jan 1 1981
Externally publishedYes

Fingerprint

Trophoblasts
Detergents
Blood Proteins
Glycoproteins
Membrane Proteins
Cell Membrane
Wheat Germ Agglutinins
Deoxycholic Acid
Membranes
Acetylglucosamine
Microvilli
Sodium Dodecyl Sulfate
Sepharose
Allografts
Polyacrylamide Gel Electrophoresis
Mothers
Peptides

ASJC Scopus subject areas

  • Immunology
  • Reproductive Medicine

Cite this

Biochemical studies of human syncytiotrophoblastic plasma membrane proteins. / O'Sullivan, M. J.; McIntyre, J. A.; Warriner, G.; Page Faulk, W.

In: Journal of Reproductive Immunology, Vol. 3, No. 5, 01.01.1981, p. 297-306.

Research output: Contribution to journalArticle

O'Sullivan, M. J. ; McIntyre, J. A. ; Warriner, G. ; Page Faulk, W. / Biochemical studies of human syncytiotrophoblastic plasma membrane proteins. In: Journal of Reproductive Immunology. 1981 ; Vol. 3, No. 5. pp. 297-306.
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