TY - JOUR
T1 - Biochemical Identity and Characterization of the Mouse Interleukin-2 Receptor β and γc Subunits
AU - Malek, Thomas R.
AU - Furse, Robert K.
AU - Fleming, Margaret L.
AU - Fadell, Anthony J.
AU - Wen, You
PY - 1995/5
Y1 - 1995/5
N2 - Although the mouse IL-2 receptor (IL-2R) β and γc subunits have been identified by molecular cloning, the biochemical identity of these subunits has not yet been established. In the present study, the mouse IL-2R was biochemically characterized from cell lines expressing normal and aberrant IL-2R. Using novel monoclonal antibodies specific for the β or γc subunits, we established that the Mr of the β chain is 90,000–100,000 and that of the γc subunit is 75,000–80,000. Analysis of transfected EL4 cells that expressed α, γc, and truncated β subunits or mutant EL4 cells, which selectively lacked cell surface γc, revealed that no other material migrated to a position on SDS-PAGE characteristic of IL-2/IL-2Rβ and IL-2/IL-2Rγc cross-linked complexes, respectively. Thus, the β and γc subunits appear to be the sole IL-2R constituents of these IL-2 cross-linked complexes. The IL-2/IL-2Rγc, but not the IL-2/IL-2Rβ, complex exhibited enhanced mobility after SDS-polyacrylamide gel electrophoresis under nonreducing conditions, suggesting a more compact structure for γc as a result of intrachain disulfide bonds. The primary posttranslational modification of the mouse β and γc subunits is N-linked glycosylation. These biochemical studies reconcile past uncertainties concerning the subunit composition of the mouse IL-2R and are consistent with a model of the IL-2R containing only three subunits.
AB - Although the mouse IL-2 receptor (IL-2R) β and γc subunits have been identified by molecular cloning, the biochemical identity of these subunits has not yet been established. In the present study, the mouse IL-2R was biochemically characterized from cell lines expressing normal and aberrant IL-2R. Using novel monoclonal antibodies specific for the β or γc subunits, we established that the Mr of the β chain is 90,000–100,000 and that of the γc subunit is 75,000–80,000. Analysis of transfected EL4 cells that expressed α, γc, and truncated β subunits or mutant EL4 cells, which selectively lacked cell surface γc, revealed that no other material migrated to a position on SDS-PAGE characteristic of IL-2/IL-2Rβ and IL-2/IL-2Rγc cross-linked complexes, respectively. Thus, the β and γc subunits appear to be the sole IL-2R constituents of these IL-2 cross-linked complexes. The IL-2/IL-2Rγc, but not the IL-2/IL-2Rβ, complex exhibited enhanced mobility after SDS-polyacrylamide gel electrophoresis under nonreducing conditions, suggesting a more compact structure for γc as a result of intrachain disulfide bonds. The primary posttranslational modification of the mouse β and γc subunits is N-linked glycosylation. These biochemical studies reconcile past uncertainties concerning the subunit composition of the mouse IL-2R and are consistent with a model of the IL-2R containing only three subunits.
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U2 - 10.1089/jir.1995.15.447
DO - 10.1089/jir.1995.15.447
M3 - Article
C2 - 7648447
AN - SCOPUS:0029005081
VL - 15
SP - 447
EP - 454
JO - Journal of Interferon and Cytokine Research
JF - Journal of Interferon and Cytokine Research
SN - 1079-9907
IS - 5
ER -