Biochemical characterization of the third domain from Bacillus thuringiensis Cry1A toxins

Robert I. Vázquez-Padrón, Ariel F. Martínez-Gil, Camilo Ayra-Pardo, Joel González-Cabrera, Dmitri L. Prieto-Samsonov, Gustavo A. De La Riva

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Cry proteins from Bacillus thuringiensis have insecticidal properties. The function of domains I and II has been described but domain III has so far eluded understanding. Domain III from Cry1Ab and Cry1Ac has been cloned, expressed in E. coli and injected to rabbits with the aid of characterizing them immunologically. Interestingly, polyclonal antibodies against Cry1Ab fragment did not recognize either the native Cry1Ab toxin or the Cry1Ac fragment while those against the latter did recognize either the native Cry1Ac toxin or the Cry1Ab protein fragment. A combination of information from sequence comparison and hydrophobicity profile indicates that these protein fragments possibly adopt different spatial dispositions within the respective toxins.

Original languageEnglish (US)
Pages (from-to)1011-1020
Number of pages10
JournalBiochemistry and Molecular Biology International
Issue number5
StatePublished - Aug 1998
Externally publishedYes


  • δ-endotoxins
  • Bacillus thuringiensis
  • Cry
  • Recombinant proteins

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology


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