Biochemical and functional antigenic mimicry by a polyclonal anti-ldiotypic antibody for chlamydial exoglycolipid antigen

Ling Ling An, Alan P. Hudson, Robert A. Prendergast, Terrence P.O. Brien, Elizabeth S. Stuart, Judith A. Whittum-Hudson, A. Bruce Mac Donald

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A vaccine developed by using a genus-specific antigen (Ag) of Chlamydia trachomatis would elicit a wide range of protection against various chlamydial infections. We have produced an anti-idiotypic antibody (Ab2) in guinea pigs which, in rabbits, mimics the immunogenicity of a genus-specific exoglycolipid Ag (GLXA) of C. trachomatis. Furthermore, the Ab2 fulfills the functional criteria of an 'internal image' of the nominal Ag: it inhibits the binding of the idiotypic (Id) monoclonal Ab (mAb1) to GLXA, and it induces in rabbits anti-anti-Id antibody (Ab3) which recognizes both the affinity-purified nominal Ag GLXA and whole organisms. Moreover, Ab3 induced by immunization of rabbits with guinea pig Ab2 neutralizes infectious heterologous chlamydiae and prevents in vitro and in vivo infection. Taken together, these results demonstrate functional and biochemical mimicry of the Ab2 for the chlamydial GLXA and suggest that anti-idiotypic Ab to GLXA is a potential candidate vaccine against chlamydia-related diseases.

Original languageEnglish (US)
Pages (from-to)229-240
Number of pages12
JournalPathobiology
Volume65
Issue number5
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

Keywords

  • Anti-anti-idiotype
  • Anti-Id antibody
  • Chlamydia trachomatis
  • Exoglycolipid
  • Idiotype
  • Neutralization
  • Trachoma
  • Vaccine

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Biochemistry
  • Immunology and Allergy
  • Cell Biology

Fingerprint Dive into the research topics of 'Biochemical and functional antigenic mimicry by a polyclonal anti-ldiotypic antibody for chlamydial exoglycolipid antigen'. Together they form a unique fingerprint.

Cite this