Binding of tRNA nucleotidyltransferase to Affi-Gel blue: Rapid purification of the enzyme and binding studies

Murray P. Deutscher, Piotr Masiakowski

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Rabbit liver tRNA nucleotidyltransferase bound to columns of Affi-Gel Blue and could be specifically eluted with tRNA. This observation led to development of a rapid purification procedure for the enzyme. The adsorbent was also used to assess interaction of tRNA nucleotidyltransferase with various polynucleotides and substrates. The enzyme could be efficiently desorbed from Affi-Gel Blue by low concentrations of tRNA-C-C, less well by tRNA-C-C-A, and not at all by poly(A), poly(C), ATP or CTP.

Original languageEnglish (US)
Pages (from-to)1947-1954
Number of pages8
JournalNucleic acids research
Volume5
Issue number6
DOIs
StatePublished - Jun 1978

ASJC Scopus subject areas

  • Genetics

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