Binding of tRNA nucleotidyltransferase to Affi-Gel blue: Rapid purification of the enzyme and binding studies

Murray P. Deutscher; Piotr Masiakowski

doi:10.1093/nar/5.6.1947

Binding of tRNA nucleotidyltransferase to Affi-Gel blue: Rapid purification of the enzyme and binding studies

Murray P. Deutscher, Piotr Masiakowski

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Rabbit liver tRNA nucleotidyltransferase bound to columns of Affi-Gel Blue and could be specifically eluted with tRNA. This observation led to development of a rapid purification procedure for the enzyme. The adsorbent was also used to assess interaction of tRNA nucleotidyltransferase with various polynucleotides and substrates. The enzyme could be efficiently desorbed from Affi-Gel Blue by low concentrations of tRNA-C-C, less well by tRNA-C-C-A, and not at all by poly(A), poly(C), ATP or CTP.

Original language	English (US)
Pages (from-to)	1947-1954
Number of pages	8
Journal	Nucleic acids research
Volume	5
Issue number	6
DOIs	https://doi.org/10.1093/nar/5.6.1947
State	Published - Jun 1978

ASJC Scopus subject areas

Genetics

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abstract = "Rabbit liver tRNA nucleotidyltransferase bound to columns of Affi-Gel Blue and could be specifically eluted with tRNA. This observation led to development of a rapid purification procedure for the enzyme. The adsorbent was also used to assess interaction of tRNA nucleotidyltransferase with various polynucleotides and substrates. The enzyme could be efficiently desorbed from Affi-Gel Blue by low concentrations of tRNA-C-C, less well by tRNA-C-C-A, and not at all by poly(A), poly(C), ATP or CTP.",

author = "Deutscher, {Murray P.} and Piotr Masiakowski",

year = "1978",

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doi = "10.1093/nar/5.6.1947",

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AU - Masiakowski, Piotr

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N2 - Rabbit liver tRNA nucleotidyltransferase bound to columns of Affi-Gel Blue and could be specifically eluted with tRNA. This observation led to development of a rapid purification procedure for the enzyme. The adsorbent was also used to assess interaction of tRNA nucleotidyltransferase with various polynucleotides and substrates. The enzyme could be efficiently desorbed from Affi-Gel Blue by low concentrations of tRNA-C-C, less well by tRNA-C-C-A, and not at all by poly(A), poly(C), ATP or CTP.

AB - Rabbit liver tRNA nucleotidyltransferase bound to columns of Affi-Gel Blue and could be specifically eluted with tRNA. This observation led to development of a rapid purification procedure for the enzyme. The adsorbent was also used to assess interaction of tRNA nucleotidyltransferase with various polynucleotides and substrates. The enzyme could be efficiently desorbed from Affi-Gel Blue by low concentrations of tRNA-C-C, less well by tRNA-C-C-A, and not at all by poly(A), poly(C), ATP or CTP.

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