TY - JOUR
T1 - Binding of tRNA nucleotidyltransferase to Affi-Gel blue
T2 - Rapid purification of the enzyme and binding studies
AU - Deutscher, Murray P.
AU - Masiakowski, Piotr
N1 - Funding Information:
This work was supported by NIH grant GM 16317. assistance of Robert McGann is greatly appreciated.
PY - 1978/6
Y1 - 1978/6
N2 - Rabbit liver tRNA nucleotidyltransferase bound to columns of Affi-Gel Blue and could be specifically eluted with tRNA. This observation led to development of a rapid purification procedure for the enzyme. The adsorbent was also used to assess interaction of tRNA nucleotidyltransferase with various polynucleotides and substrates. The enzyme could be efficiently desorbed from Affi-Gel Blue by low concentrations of tRNA-C-C, less well by tRNA-C-C-A, and not at all by poly(A), poly(C), ATP or CTP.
AB - Rabbit liver tRNA nucleotidyltransferase bound to columns of Affi-Gel Blue and could be specifically eluted with tRNA. This observation led to development of a rapid purification procedure for the enzyme. The adsorbent was also used to assess interaction of tRNA nucleotidyltransferase with various polynucleotides and substrates. The enzyme could be efficiently desorbed from Affi-Gel Blue by low concentrations of tRNA-C-C, less well by tRNA-C-C-A, and not at all by poly(A), poly(C), ATP or CTP.
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U2 - 10.1093/nar/5.6.1947
DO - 10.1093/nar/5.6.1947
M3 - Article
C2 - 673841
AN - SCOPUS:0017850670
VL - 5
SP - 1947
EP - 1954
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 6
ER -