Abstract
The purpose of this study was to determine whether transient unilateral (2 h) middle cerebral artery occlusion (MCAo) is capable of inducing bilateral ischemic tolerance in hippocampal CA1 neurons when temporary bilateral forebrain ischemia by two-vessel occlusion (2VO) is carried out 3 days later, and to explore the relationship of this tolerance to the regional expression of c-fos and hsp-70 mRNA. Rats were sacrificed 4 days after 2VO, and normal-appearing neurons in CA1 subregions were counted. Rats subjected to MCAo and 2VO showed significant protection of CA1 neurons in both hippocampi, whereas rats which underwent sham MCAo and 2VO typically had severe bilateral destruction of CA1 neurons (normal neuron counts, ipsilateral medial CA1: 59.8 ± 7.2 vs 16.6 ± 7.8 (mean ± s.e.m.); middle CA1: 50.0 ± 4.7 vs 16.0 ± 8.8; lateral CA1: 43.5 ± 5.7 vs 13.8 ± 6.3; contralateral, medial CA1: 52.3 ± 6.3 vs 17.0 ± 6.4; middle CA1: 43.3 ± 4.7 vs 19.8 ± 8.1; lateral CA1: 45.5 ± 4.6 vs 26.0 ± 10.3, respectively). This neuronal tolerance was preceded by the early bilateral induction of c-fos mRNA, which may in turn lead to expression of critical target genes that promote cell recovery.
| Original language | English |
|---|---|
| Pages (from-to) | 55-59 |
| Number of pages | 5 |
| Journal | NeuroReport |
| Volume | 8 |
| Issue number | 1 |
| State | Published - May 12 1997 |
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Keywords
- Bilateral carotid artery occlusion
- In situ hybridization
- Middle cerebral artery occlusion
- Selective vulnerability
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Bilateral ischemic tolerance of rat hippocampus induced by prior unilateral transient focal ischemia : Relationship to c-fos mRNA expression. / Belayev, Ludmila; Ginsberg, Myron; Alonso, Ofelia F.; Singer, Jessie T.; Zhao, Weizhao; Busto, Raul.
In: NeuroReport, Vol. 8, No. 1, 12.05.1997, p. 55-59.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bilateral ischemic tolerance of rat hippocampus induced by prior unilateral transient focal ischemia
T2 - Relationship to c-fos mRNA expression
AU - Belayev, Ludmila
AU - Ginsberg, Myron
AU - Alonso, Ofelia F.
AU - Singer, Jessie T.
AU - Zhao, Weizhao
AU - Busto, Raul
PY - 1997/5/12
Y1 - 1997/5/12
N2 - The purpose of this study was to determine whether transient unilateral (2 h) middle cerebral artery occlusion (MCAo) is capable of inducing bilateral ischemic tolerance in hippocampal CA1 neurons when temporary bilateral forebrain ischemia by two-vessel occlusion (2VO) is carried out 3 days later, and to explore the relationship of this tolerance to the regional expression of c-fos and hsp-70 mRNA. Rats were sacrificed 4 days after 2VO, and normal-appearing neurons in CA1 subregions were counted. Rats subjected to MCAo and 2VO showed significant protection of CA1 neurons in both hippocampi, whereas rats which underwent sham MCAo and 2VO typically had severe bilateral destruction of CA1 neurons (normal neuron counts, ipsilateral medial CA1: 59.8 ± 7.2 vs 16.6 ± 7.8 (mean ± s.e.m.); middle CA1: 50.0 ± 4.7 vs 16.0 ± 8.8; lateral CA1: 43.5 ± 5.7 vs 13.8 ± 6.3; contralateral, medial CA1: 52.3 ± 6.3 vs 17.0 ± 6.4; middle CA1: 43.3 ± 4.7 vs 19.8 ± 8.1; lateral CA1: 45.5 ± 4.6 vs 26.0 ± 10.3, respectively). This neuronal tolerance was preceded by the early bilateral induction of c-fos mRNA, which may in turn lead to expression of critical target genes that promote cell recovery.
AB - The purpose of this study was to determine whether transient unilateral (2 h) middle cerebral artery occlusion (MCAo) is capable of inducing bilateral ischemic tolerance in hippocampal CA1 neurons when temporary bilateral forebrain ischemia by two-vessel occlusion (2VO) is carried out 3 days later, and to explore the relationship of this tolerance to the regional expression of c-fos and hsp-70 mRNA. Rats were sacrificed 4 days after 2VO, and normal-appearing neurons in CA1 subregions were counted. Rats subjected to MCAo and 2VO showed significant protection of CA1 neurons in both hippocampi, whereas rats which underwent sham MCAo and 2VO typically had severe bilateral destruction of CA1 neurons (normal neuron counts, ipsilateral medial CA1: 59.8 ± 7.2 vs 16.6 ± 7.8 (mean ± s.e.m.); middle CA1: 50.0 ± 4.7 vs 16.0 ± 8.8; lateral CA1: 43.5 ± 5.7 vs 13.8 ± 6.3; contralateral, medial CA1: 52.3 ± 6.3 vs 17.0 ± 6.4; middle CA1: 43.3 ± 4.7 vs 19.8 ± 8.1; lateral CA1: 45.5 ± 4.6 vs 26.0 ± 10.3, respectively). This neuronal tolerance was preceded by the early bilateral induction of c-fos mRNA, which may in turn lead to expression of critical target genes that promote cell recovery.
KW - Bilateral carotid artery occlusion
KW - In situ hybridization
KW - Middle cerebral artery occlusion
KW - Selective vulnerability
UR - http://www.scopus.com/inward/record.url?scp=0030967145&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030967145&partnerID=8YFLogxK
M3 - Article
VL - 8
SP - 55
EP - 59
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 1
ER -