Bidirectional regulation of HDAC5 by mAKAPβ signalosomes in cardiac myocytes

Kimberly L. Dodge-Kafka, Moriah Gildart, Jinliang Li, Hrishikesh Thakur, Michael S Kapiloff

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Class IIa histone deacetylases (HDACs) are transcriptional repressors whose nuclear export in the cardiac myocyte is associated with the induction of pathological gene expression and cardiac remodeling. Class IIa HDACs are regulated by multiple, functionally opposing post-translational modifications, including phosphorylation by protein kinase D (PKD) that promotes nuclear export and phosphorylation by protein kinase A (PKA) that promotes nuclear import. We have previously shown that the scaffold protein muscle A-kinase anchoring protein β (mAKAPβ) orchestrates signaling in the cardiac myocyte required for pathological cardiac remodeling, including serving as a scaffold for both PKD and PKA. We now show that mAKAPβ is a scaffold for HDAC5 in cardiac myocytes, forming signalosomes containing HDAC5, PKD, and PKA. Inhibition of mAKAPβ expression attenuated the phosphorylation of HDAC5 by PKD and PKA in response to α- and β-adrenergic receptor stimulation, respectively. Importantly, disruption of mAKAPβ-HDAC5 anchoring prevented the induction of HDAC5 nuclear export by α-adrenergic receptor signaling and PKD phosphorylation. In addition, disruption of mAKAPβ-PKA anchoring prevented the inhibition by β-adrenergic receptor stimulation of α-adrenergic-induced HDAC5 nuclear export. Together, these data establish that mAKAPβ signalosomes serve to bidirectionally regulate the nuclear-cytoplasmic localization of class IIa HDACs. Thus, the mAKAPβ scaffold serves as a node in the myocyte regulatory network controlling both the repression and activation of pathological gene expression in health and disease, respectively.

Original languageEnglish (US)
Pages (from-to)13-25
Number of pages13
JournalJournal of Molecular and Cellular Cardiology
Volume118
DOIs
StatePublished - May 1 2018
Externally publishedYes

Fingerprint

Cardiac Myocytes
Protein Kinases
Cell Nucleus Active Transport
Cyclic AMP-Dependent Protein Kinases
Muscles
Histone Deacetylases
Phosphorylation
Adrenergic Receptors
Gene Expression
Muscle Proteins
Post Translational Protein Processing
Adrenergic Agents
Muscle Cells
protein kinase D
Health

Keywords

  • HDAC5
  • mAKAP
  • Phosphorylation
  • PKA
  • PKD
  • Scaffold

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Bidirectional regulation of HDAC5 by mAKAPβ signalosomes in cardiac myocytes. / Dodge-Kafka, Kimberly L.; Gildart, Moriah; Li, Jinliang; Thakur, Hrishikesh; Kapiloff, Michael S.

In: Journal of Molecular and Cellular Cardiology, Vol. 118, 01.05.2018, p. 13-25.

Research output: Contribution to journalArticle

Dodge-Kafka, Kimberly L. ; Gildart, Moriah ; Li, Jinliang ; Thakur, Hrishikesh ; Kapiloff, Michael S. / Bidirectional regulation of HDAC5 by mAKAPβ signalosomes in cardiac myocytes. In: Journal of Molecular and Cellular Cardiology. 2018 ; Vol. 118. pp. 13-25.
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