BFGF-containing electrospun gelatin scaffolds with controlled nano-architectural features for directed angiogenesis

Ramon Montero, Ximena Vial, Dat Tat Nguyen, Sepehr Farhand, Mark Reardon, Si M. Pham, Gavriil Tsechpenakis, Fotios M Andreopoulos

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Current therapeutic angiogenesis strategies are focused on the development of biologically responsive scaffolds that can deliver multiple angiogenic cytokines and/or cells in ischemic regions. Herein, we report on a novel electrospinning approach to fabricate cytokine-containing nanofibrous scaffolds with tunable architecture to promote angiogenesis. Fiber diameter and uniformity were controlled by varying the concentration of the polymeric (i.e. gelatin) solution, the feed rate, needle to collector distance, and electric field potential between the collector plate and injection needle. Scaffold fiber orientation (random vs. aligned) was achieved by alternating the polarity of two parallel electrodes placed on the collector plate thus dictating fiber deposition patterns. Basic fibroblast growth factor (bFGF) was physically immobilized within the gelatin scaffolds at variable concentrations and human umbilical vein endothelial cells (HUVEC) were seeded on the top of the scaffolds. Cell proliferation and migration was assessed as a function of growth factor loading and scaffold architecture. HUVECs successfully adhered onto gelatin B scaffolds and cell proliferation was directly proportional to the loading concentrations of the growth factor (0-100 bFGF ng/mL). Fiber orientation had a pronounced effect on cell morphology and orientation. Cells were spread along the fibers of the electrospun scaffolds with the aligned orientation and developed a spindle-like morphology parallel to the scaffold's fibers. In contrast, cells seeded onto the scaffolds with random fiber orientation, did not demonstrate any directionality and appeared to have a rounder shape. Capillary formation (i.e. sprouts length and number of sprouts per bead), assessed in a 3-D in vitro angiogenesis assay, was a function of bFGF loading concentration (0 ng, 50 ng and 100 ng per scaffold) for both types of electrospun scaffolds (i.e. with aligned or random fiber orientation).

Original languageEnglish
Pages (from-to)1778-1791
Number of pages14
JournalActa Biomaterialia
Volume8
Issue number5
DOIs
StatePublished - May 1 2012

Fingerprint

Gelatin
Scaffolds
Fibroblast Growth Factor 2
Needles
Intercellular Signaling Peptides and Proteins
Fiber reinforced materials
Cell Proliferation
Cytokines
Fibroblasts
Human Umbilical Vein Endothelial Cells
Fibers
Cell Movement
Cell proliferation
Scaffolds (biology)
Electrodes
Injections
Endothelial cells
Electrospinning
Assays
Electric fields

Keywords

  • Angiogenesis
  • BFGF
  • Cell orientation
  • Electrospinning
  • Scaffolds

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

BFGF-containing electrospun gelatin scaffolds with controlled nano-architectural features for directed angiogenesis. / Montero, Ramon; Vial, Ximena; Nguyen, Dat Tat; Farhand, Sepehr; Reardon, Mark; Pham, Si M.; Tsechpenakis, Gavriil; Andreopoulos, Fotios M.

In: Acta Biomaterialia, Vol. 8, No. 5, 01.05.2012, p. 1778-1791.

Research output: Contribution to journalArticle

Montero, Ramon ; Vial, Ximena ; Nguyen, Dat Tat ; Farhand, Sepehr ; Reardon, Mark ; Pham, Si M. ; Tsechpenakis, Gavriil ; Andreopoulos, Fotios M. / BFGF-containing electrospun gelatin scaffolds with controlled nano-architectural features for directed angiogenesis. In: Acta Biomaterialia. 2012 ; Vol. 8, No. 5. pp. 1778-1791.
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