Beyond antibodies as binding partners: The role of antibody mimetics in bioanalysis

Xiaowen Yu, Yu Ping Yang, Emre Dikici, Sapna K. Deo, Sylvia Daunert

Research output: Contribution to journalReview articlepeer-review

24 Scopus citations


The emergence of novel binding proteins or antibody mimetics capable of binding to ligand analytes in a manner analogous to that of the antigen-antibody interaction has spurred increased interest in the biotechnology and bioanalytical communities. The goal is to produce antibody mimetics designed to outperform antibodies with regard to binding affinities, cellular and tumor penetration, large-scale production, and temperature and pH stability. The generation of antibody mimetics with tailored characteristics involves the identification of a naturally occurring protein scaffold as a template that binds to a desired ligand. This scaffold is then engineered to create a superior binder by first creating a library that is then subjected to a series of selection steps. Antibody mimetics have been successfully used in the development of binding assays for the detection of analytes in biological samples, as well as in separation methods, cancer therapy, targeted drug delivery, and in vivo imaging. This review describes recent advances in the field of antibody mimetics and their applications in bioanalytical chemistry, specifically in diagnostics and other analytical methods.

Original languageEnglish (US)
Pages (from-to)293-320
Number of pages28
JournalAnnual Review of Analytical Chemistry
StatePublished - Jun 12 2017


  • Antibodies
  • Antibody mimetics
  • Biosensors
  • Diagnostics
  • Molecular imaging
  • Protein scaffolds

ASJC Scopus subject areas

  • Analytical Chemistry


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