Abstract
Betacellulin (BTC) belongs to the EGF family, whose members play important roles in skin morphogenesis, homeostasis, and repair. However, the role of BTC in skin biology is still unknown. We employed transgenic mice overexpressing BTC ubiquitously to study its role in skin physiology. Immunohistochemistry revealed increased levels of BTC especially in the hair follicles and in the epidermis of transgenic animals. Expression of key markers of epithelial differentiation was unaltered, but keratinocyte proliferation was significantly increased. At post-natal day 1 (P1), transgenic mice displayed a significant retardation of hair follicle morphogenesis. At P17, when most follicles in control mice had initiated hair follicle cycling and had already entered into their first late catagen or telogen phase, all follicles of transgenic mice were still at the mid- to late catagen phases, indicating retarded initiation of hair follicle cycling. Healing of full-thickness excisional wounds and bursting strength of incisional wounds were similar in control and transgenic mice. However, an increase in the area covered by blood vessels at the wound site was detected in transgenic animals. These results provide evidence for a role of BTC in the regulation of epidermal homeostasis, hair follicle morphogenesis and cycling, and wound angiogenesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1256-1265 |
| Number of pages | 10 |
| Journal | Journal of Investigative Dermatology |
| Volume | 128 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jan 1 2008 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Dermatology
- Cell Biology
Cite this
Betacellulin regulates hair follicle development and hair cycle induction and enhances angiogenesis in wounded skin. / Schneider, Marlon R.; Antsiferova, Maria; Feldmeyer, Laurence; Dahlhoff, Maik; Bugnon, Philippe; Hasse, Sybille; Paus, Ralf; Wolf, Eckhard; Werner, Sabine.
In: Journal of Investigative Dermatology, Vol. 128, No. 5, 01.01.2008, p. 1256-1265.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Betacellulin regulates hair follicle development and hair cycle induction and enhances angiogenesis in wounded skin
AU - Schneider, Marlon R.
AU - Antsiferova, Maria
AU - Feldmeyer, Laurence
AU - Dahlhoff, Maik
AU - Bugnon, Philippe
AU - Hasse, Sybille
AU - Paus, Ralf
AU - Wolf, Eckhard
AU - Werner, Sabine
PY - 2008/1/1
Y1 - 2008/1/1
N2 - Betacellulin (BTC) belongs to the EGF family, whose members play important roles in skin morphogenesis, homeostasis, and repair. However, the role of BTC in skin biology is still unknown. We employed transgenic mice overexpressing BTC ubiquitously to study its role in skin physiology. Immunohistochemistry revealed increased levels of BTC especially in the hair follicles and in the epidermis of transgenic animals. Expression of key markers of epithelial differentiation was unaltered, but keratinocyte proliferation was significantly increased. At post-natal day 1 (P1), transgenic mice displayed a significant retardation of hair follicle morphogenesis. At P17, when most follicles in control mice had initiated hair follicle cycling and had already entered into their first late catagen or telogen phase, all follicles of transgenic mice were still at the mid- to late catagen phases, indicating retarded initiation of hair follicle cycling. Healing of full-thickness excisional wounds and bursting strength of incisional wounds were similar in control and transgenic mice. However, an increase in the area covered by blood vessels at the wound site was detected in transgenic animals. These results provide evidence for a role of BTC in the regulation of epidermal homeostasis, hair follicle morphogenesis and cycling, and wound angiogenesis.
AB - Betacellulin (BTC) belongs to the EGF family, whose members play important roles in skin morphogenesis, homeostasis, and repair. However, the role of BTC in skin biology is still unknown. We employed transgenic mice overexpressing BTC ubiquitously to study its role in skin physiology. Immunohistochemistry revealed increased levels of BTC especially in the hair follicles and in the epidermis of transgenic animals. Expression of key markers of epithelial differentiation was unaltered, but keratinocyte proliferation was significantly increased. At post-natal day 1 (P1), transgenic mice displayed a significant retardation of hair follicle morphogenesis. At P17, when most follicles in control mice had initiated hair follicle cycling and had already entered into their first late catagen or telogen phase, all follicles of transgenic mice were still at the mid- to late catagen phases, indicating retarded initiation of hair follicle cycling. Healing of full-thickness excisional wounds and bursting strength of incisional wounds were similar in control and transgenic mice. However, an increase in the area covered by blood vessels at the wound site was detected in transgenic animals. These results provide evidence for a role of BTC in the regulation of epidermal homeostasis, hair follicle morphogenesis and cycling, and wound angiogenesis.
UR - http://www.scopus.com/inward/record.url?scp=42149099334&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42149099334&partnerID=8YFLogxK
U2 - 10.1038/sj.jid.5701135
DO - 10.1038/sj.jid.5701135
M3 - Article
C2 - 17960175
AN - SCOPUS:42149099334
VL - 128
SP - 1256
EP - 1265
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 5
ER -