Ber-EP4 immunoreactivity in normal skin and cutaneous neoplasms

F. J. Jimenez, J. L. Burchette, James M Grichnik, M. G. Hitchcock

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Ber-EP4 is an antibody raised against a cell membrane glycoprotein of, as yet, unknown function. In the skin, the ability to distinguish basal cell carcinoma from squamous cell carcinoma has been emphasized. Immunoreactivity in apocrine and eccrine secretory coil epithelium, Merkel cell carcinoma, and cutaneous mixed tumor has been reported. Having observed more widespread staining than previously reported, we sought to characterize further Ber-EP4 immunoreactivity by examining 76 formalin-fixed and paraffin-embedded proliferative epithelial skin lesions, 2 sections of fetal skin with developing adnexa, and frozen sections of skin from Mohs surgery procedures, using hematoxylin and eosin and Ber-EP4 immunostaining. In paraffin-embedded skin, matrical and outer sheath epithelium of vellus anagen follicles, inferior segment epithelium of vellus telogen follicles, and secretory coils of sweat glands stain with Ber-EP4, but anagen terminal follicle epithelium and eccrine and apocrine sweat ducts are negative. On frozen section, additional staining of eccrine ducts and cells in the outer sheath and matrix of the inferior segment of terminal follicles is noted. Basaloid epithelium of trichoepitheliomas, follicular induction over dermatofibromas, mammary Paget's disease, and selected other neoplasms showing eccrine or apocrine differentiation stain with Ber-EP4. These data indicate that in addition to basal cell carcinoma, Ber-EP4 staining may be seen in a variety of proliferative epidermal and adnexal lesions.

Original languageEnglish
Pages (from-to)854-858
Number of pages5
JournalModern Pathology
Volume8
Issue number8
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Skin Neoplasms
Epithelium
Skin
Basal Cell Carcinoma
Frozen Sections
Staining and Labeling
Paraffin
Coloring Agents
Mammary Paget's Disease
Mohs Surgery
Dermatologic Surgical Procedures
Benign Fibrous Histiocytoma
Merkel Cell Carcinoma
Sweat Glands
Sweat
Membrane Glycoproteins
Hematoxylin
Eosine Yellowish-(YS)
Formaldehyde
Squamous Cell Carcinoma

Keywords

  • Adnexal
  • Ber-EP4
  • Immunohistochemistry
  • Neoplasms
  • Skin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Jimenez, F. J., Burchette, J. L., Grichnik, J. M., & Hitchcock, M. G. (1995). Ber-EP4 immunoreactivity in normal skin and cutaneous neoplasms. Modern Pathology, 8(8), 854-858.

Ber-EP4 immunoreactivity in normal skin and cutaneous neoplasms. / Jimenez, F. J.; Burchette, J. L.; Grichnik, James M; Hitchcock, M. G.

In: Modern Pathology, Vol. 8, No. 8, 01.01.1995, p. 854-858.

Research output: Contribution to journalArticle

Jimenez, FJ, Burchette, JL, Grichnik, JM & Hitchcock, MG 1995, 'Ber-EP4 immunoreactivity in normal skin and cutaneous neoplasms', Modern Pathology, vol. 8, no. 8, pp. 854-858.
Jimenez FJ, Burchette JL, Grichnik JM, Hitchcock MG. Ber-EP4 immunoreactivity in normal skin and cutaneous neoplasms. Modern Pathology. 1995 Jan 1;8(8):854-858.
Jimenez, F. J. ; Burchette, J. L. ; Grichnik, James M ; Hitchcock, M. G. / Ber-EP4 immunoreactivity in normal skin and cutaneous neoplasms. In: Modern Pathology. 1995 ; Vol. 8, No. 8. pp. 854-858.
@article{e1246536060a4b50be1ca7d5959f9b2f,
title = "Ber-EP4 immunoreactivity in normal skin and cutaneous neoplasms",
abstract = "Ber-EP4 is an antibody raised against a cell membrane glycoprotein of, as yet, unknown function. In the skin, the ability to distinguish basal cell carcinoma from squamous cell carcinoma has been emphasized. Immunoreactivity in apocrine and eccrine secretory coil epithelium, Merkel cell carcinoma, and cutaneous mixed tumor has been reported. Having observed more widespread staining than previously reported, we sought to characterize further Ber-EP4 immunoreactivity by examining 76 formalin-fixed and paraffin-embedded proliferative epithelial skin lesions, 2 sections of fetal skin with developing adnexa, and frozen sections of skin from Mohs surgery procedures, using hematoxylin and eosin and Ber-EP4 immunostaining. In paraffin-embedded skin, matrical and outer sheath epithelium of vellus anagen follicles, inferior segment epithelium of vellus telogen follicles, and secretory coils of sweat glands stain with Ber-EP4, but anagen terminal follicle epithelium and eccrine and apocrine sweat ducts are negative. On frozen section, additional staining of eccrine ducts and cells in the outer sheath and matrix of the inferior segment of terminal follicles is noted. Basaloid epithelium of trichoepitheliomas, follicular induction over dermatofibromas, mammary Paget's disease, and selected other neoplasms showing eccrine or apocrine differentiation stain with Ber-EP4. These data indicate that in addition to basal cell carcinoma, Ber-EP4 staining may be seen in a variety of proliferative epidermal and adnexal lesions.",
keywords = "Adnexal, Ber-EP4, Immunohistochemistry, Neoplasms, Skin",
author = "Jimenez, {F. J.} and Burchette, {J. L.} and Grichnik, {James M} and Hitchcock, {M. G.}",
year = "1995",
month = "1",
day = "1",
language = "English",
volume = "8",
pages = "854--858",
journal = "Modern Pathology",
issn = "0893-3952",
publisher = "Nature Publishing Group",
number = "8",

}

TY - JOUR

T1 - Ber-EP4 immunoreactivity in normal skin and cutaneous neoplasms

AU - Jimenez, F. J.

AU - Burchette, J. L.

AU - Grichnik, James M

AU - Hitchcock, M. G.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Ber-EP4 is an antibody raised against a cell membrane glycoprotein of, as yet, unknown function. In the skin, the ability to distinguish basal cell carcinoma from squamous cell carcinoma has been emphasized. Immunoreactivity in apocrine and eccrine secretory coil epithelium, Merkel cell carcinoma, and cutaneous mixed tumor has been reported. Having observed more widespread staining than previously reported, we sought to characterize further Ber-EP4 immunoreactivity by examining 76 formalin-fixed and paraffin-embedded proliferative epithelial skin lesions, 2 sections of fetal skin with developing adnexa, and frozen sections of skin from Mohs surgery procedures, using hematoxylin and eosin and Ber-EP4 immunostaining. In paraffin-embedded skin, matrical and outer sheath epithelium of vellus anagen follicles, inferior segment epithelium of vellus telogen follicles, and secretory coils of sweat glands stain with Ber-EP4, but anagen terminal follicle epithelium and eccrine and apocrine sweat ducts are negative. On frozen section, additional staining of eccrine ducts and cells in the outer sheath and matrix of the inferior segment of terminal follicles is noted. Basaloid epithelium of trichoepitheliomas, follicular induction over dermatofibromas, mammary Paget's disease, and selected other neoplasms showing eccrine or apocrine differentiation stain with Ber-EP4. These data indicate that in addition to basal cell carcinoma, Ber-EP4 staining may be seen in a variety of proliferative epidermal and adnexal lesions.

AB - Ber-EP4 is an antibody raised against a cell membrane glycoprotein of, as yet, unknown function. In the skin, the ability to distinguish basal cell carcinoma from squamous cell carcinoma has been emphasized. Immunoreactivity in apocrine and eccrine secretory coil epithelium, Merkel cell carcinoma, and cutaneous mixed tumor has been reported. Having observed more widespread staining than previously reported, we sought to characterize further Ber-EP4 immunoreactivity by examining 76 formalin-fixed and paraffin-embedded proliferative epithelial skin lesions, 2 sections of fetal skin with developing adnexa, and frozen sections of skin from Mohs surgery procedures, using hematoxylin and eosin and Ber-EP4 immunostaining. In paraffin-embedded skin, matrical and outer sheath epithelium of vellus anagen follicles, inferior segment epithelium of vellus telogen follicles, and secretory coils of sweat glands stain with Ber-EP4, but anagen terminal follicle epithelium and eccrine and apocrine sweat ducts are negative. On frozen section, additional staining of eccrine ducts and cells in the outer sheath and matrix of the inferior segment of terminal follicles is noted. Basaloid epithelium of trichoepitheliomas, follicular induction over dermatofibromas, mammary Paget's disease, and selected other neoplasms showing eccrine or apocrine differentiation stain with Ber-EP4. These data indicate that in addition to basal cell carcinoma, Ber-EP4 staining may be seen in a variety of proliferative epidermal and adnexal lesions.

KW - Adnexal

KW - Ber-EP4

KW - Immunohistochemistry

KW - Neoplasms

KW - Skin

UR - http://www.scopus.com/inward/record.url?scp=0028803664&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028803664&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 854

EP - 858

JO - Modern Pathology

JF - Modern Pathology

SN - 0893-3952

IS - 8

ER -