Benextramine irreversibly inhibits [125I]neuropeptide Y affinity labeling of the Y2 binding protein in bovine hippocampus

W. Li, R. G. MacDonald, T. D. Hexum

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Affinity labeling of iodinated neuropeptide Y (NPY) to bovine hippocampal binding proteins revealed that benextramine inhibited specific NPY labeling of the 50 kDa NPY binding protein (Y2 binding protein) in a dose-dependent manner (IC50 = 33 M). Hippocampal membranes, which were pretreated with benextramine and washed, exhibited decreased [125I]NPY labeling of binding proteins in a similar dose-dependent manner. These findings demonstrate that benextramine irreversibly blocks specific NPY binding to the 50 kDa NPY Y2 binding protein.

Original languageEnglish (US)
Pages (from-to)89-91
Number of pages3
JournalEuropean Journal of Pharmacology: Molecular Pharmacology
Volume207
Issue number1
DOIs
StatePublished - May 25 1991
Externally publishedYes

Keywords

  • Affinity labeling
  • Benextramine
  • Hippocampus (bovine)
  • Neuropeptide Y (NPY)
  • Y binding protein

ASJC Scopus subject areas

  • Pharmacology

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