Beneficial effects of ischemic preconditioning on pancreas cold preservation

Anthony Richard Hogan, Marco Doni, Ruth Molano, Melina M. Ribeiro, Angela Szeto, Lorenzo Cobianchi, Elsie Zahr-Akrawi, Judith Molina, Alessia Fornoni, Armando J Mendez, Camillo Ricordi, Ricardo Pastori, Antonello Pileggi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Ischemic preconditioning (IPC) confers tissue resistance to subsequent ischemia in several organs. The protective effects are obtained by applying short periods of warm ischemia followed by reperfusion prior to extended ischemic insults to the organs. In the present study, we evaluated whether IPC can reduce pancreatic tissue injury following cold ischemic preservation. Rat pancreata were exposed to IPC (10 min of warm ischemia followed by 10 min of reperfusion) prior to ~ 18 h of cold preservation before assessment of organ injury or islet isolation. Pancreas IPC improved islet yields (964 ± 336 vs. 711 ± 204 IEQ/pancreas; p = 0.004) and lowered islet loss after culture (33 ± 10% vs. 51 ± 14%; p = 0.0005). Islet potency in vivo was well preserved with diabetes reversal and improved glucose clearance. Pancreas IPC reduced levels of NADPH-dependent oxidase, a source of reactive oxygen species, in pancreas homogenates versus controls (78.4 ± 45.9 vs. 216.2 ± 53.8 RLU/μg; p = 0.002). Microarray genomic analysis of pancreata revealed upregulation of 81 genes and downregulation of 454 genes (greater than twofold change) when comparing IPCtreated glands to controls, respectively, and showing a decrease in markers of apoptosis and oxidative stress. Collectively, our study demonstrates beneficial effects of IPC of the pancreas prior to cold organ preservation and provides evidence of the key role of IPC-mediated modulation of oxidative stress pathways. The use of IPC of the pancreas may contribute to increasing the quality of donor pancreas for transplantation and to improving organ utilization.

Original languageEnglish
Pages (from-to)1349-1360
Number of pages12
JournalCell Transplantation
Volume21
Issue number7
DOIs
StatePublished - Nov 12 2012

Fingerprint

Ischemic Preconditioning
Oxidative stress
Pancreas
Genes
Tissue
Cell death
Microarrays
Medical problems
Glucose
Rats
Modulation
Warm Ischemia
Oxygen
Reperfusion
Oxidative Stress
Organ Preservation
Pancreas Transplantation
NADPH Oxidase
Microarray Analysis
Reactive Oxygen Species

Keywords

  • Cold ischemia
  • Cold preservation
  • Ischemic preconditioning
  • Islets of Langerhans
  • Microarrays
  • NADPH oxidase
  • NADPH-dependent superoxide (NOX)
  • Oxidative stress
  • Pancreas
  • Rat
  • Transcriptome

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation
  • Biomedical Engineering

Cite this

Beneficial effects of ischemic preconditioning on pancreas cold preservation. / Hogan, Anthony Richard; Doni, Marco; Molano, Ruth; Ribeiro, Melina M.; Szeto, Angela; Cobianchi, Lorenzo; Zahr-Akrawi, Elsie; Molina, Judith; Fornoni, Alessia; Mendez, Armando J; Ricordi, Camillo; Pastori, Ricardo; Pileggi, Antonello.

In: Cell Transplantation, Vol. 21, No. 7, 12.11.2012, p. 1349-1360.

Research output: Contribution to journalArticle

Hogan, Anthony Richard ; Doni, Marco ; Molano, Ruth ; Ribeiro, Melina M. ; Szeto, Angela ; Cobianchi, Lorenzo ; Zahr-Akrawi, Elsie ; Molina, Judith ; Fornoni, Alessia ; Mendez, Armando J ; Ricordi, Camillo ; Pastori, Ricardo ; Pileggi, Antonello. / Beneficial effects of ischemic preconditioning on pancreas cold preservation. In: Cell Transplantation. 2012 ; Vol. 21, No. 7. pp. 1349-1360.
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AU - Molina, Judith

AU - Fornoni, Alessia

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