Bcl10 expression, rearrangement and mutation in MALT lymphoma: correlation with expression of nuclear factor-kappaB.

K. Ohshima, H. Muta, C. Kawasaki, K. Muta, V. Deyev, M. Kanda, Y. Kumano, E. R. Podack, M. Kikuchi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Mucosa-associated lymphoid tissue (MALT) lymphomas usually involve extranodal sites, especially the stomach, lung and salivary glands. The Bcl10 gene was recently isolated from the breakpoint region of t(1;14) (p22;q32) in MALT lymphomas, and considered to be an apoptosis-associated gene, and involves a caspase recruitment domain (CARD)-containing protein that activates NF-kappaB. We investigated the role of Bcl10 in MALT lymphoma by analyzing its expression, rearrangement and somatic mutation, by immunostaining, reverse transcriptase-polymerase chain reaction (RT-PCR), Southern blot and PCR in 20 cases of MALT lymphoma. Expression of NF-kappaB was studied by immunostaining. Five cases of reactive lymphadenitis (RLA) were used as the control. Bcl10 rearrangement was detected in 8 of 20 (40%) MALT lymphomas, but in none of RLA. Significant Bcl10 mutation was detected only in 1 case (5%) with MALT, but not in RLA. RT-PCR showed higher density bands of Bcl10 in MALT lymphomas than in RLA. Immunostaining showed a weak Bcl10 expression in the germinal center and very weak expression in the marginal zone B-cells in RLA, which was limited to the cytoplasm. In contrast, Bcl10 was strongly expressed in MALT lymphomas, and was mainly detected in the cytoplasm, as well as in the nuclei. Bcl10 expression did not correlate with Bcl10 mutation and re-arrangements. NF-kappaB was expressed in nuclei of MALT lymphoma cells, but not in RLA. Bcl10 expression in MALT lymphoma correlated closely with NF-kappaB expression. Our results suggest that activation of Bcl10 and NF-kappaB may be important in MALT lymphomagenesis, and that nuclear localization of Bcl10 may be important in the progression of MALT.

Original languageEnglish
Pages (from-to)283-289
Number of pages7
JournalInternational Journal of Oncology
Volume19
Issue number2
StatePublished - Aug 1 2001
Externally publishedYes

Fingerprint

Marginal Zone B-Cell Lymphoma
Lymphadenitis
NF-kappa B
Mutation
Lymphoid Tissue
Mucous Membrane
Reverse Transcriptase Polymerase Chain Reaction
Cytoplasm
Germinal Center
Southern Blotting
Salivary Glands
Genes
Stomach
B-Lymphocytes
Apoptosis
Polymerase Chain Reaction
Lung

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ohshima, K., Muta, H., Kawasaki, C., Muta, K., Deyev, V., Kanda, M., ... Kikuchi, M. (2001). Bcl10 expression, rearrangement and mutation in MALT lymphoma: correlation with expression of nuclear factor-kappaB. International Journal of Oncology, 19(2), 283-289.

Bcl10 expression, rearrangement and mutation in MALT lymphoma : correlation with expression of nuclear factor-kappaB. / Ohshima, K.; Muta, H.; Kawasaki, C.; Muta, K.; Deyev, V.; Kanda, M.; Kumano, Y.; Podack, E. R.; Kikuchi, M.

In: International Journal of Oncology, Vol. 19, No. 2, 01.08.2001, p. 283-289.

Research output: Contribution to journalArticle

Ohshima, K, Muta, H, Kawasaki, C, Muta, K, Deyev, V, Kanda, M, Kumano, Y, Podack, ER & Kikuchi, M 2001, 'Bcl10 expression, rearrangement and mutation in MALT lymphoma: correlation with expression of nuclear factor-kappaB.', International Journal of Oncology, vol. 19, no. 2, pp. 283-289.
Ohshima, K. ; Muta, H. ; Kawasaki, C. ; Muta, K. ; Deyev, V. ; Kanda, M. ; Kumano, Y. ; Podack, E. R. ; Kikuchi, M. / Bcl10 expression, rearrangement and mutation in MALT lymphoma : correlation with expression of nuclear factor-kappaB. In: International Journal of Oncology. 2001 ; Vol. 19, No. 2. pp. 283-289.
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abstract = "Mucosa-associated lymphoid tissue (MALT) lymphomas usually involve extranodal sites, especially the stomach, lung and salivary glands. The Bcl10 gene was recently isolated from the breakpoint region of t(1;14) (p22;q32) in MALT lymphomas, and considered to be an apoptosis-associated gene, and involves a caspase recruitment domain (CARD)-containing protein that activates NF-kappaB. We investigated the role of Bcl10 in MALT lymphoma by analyzing its expression, rearrangement and somatic mutation, by immunostaining, reverse transcriptase-polymerase chain reaction (RT-PCR), Southern blot and PCR in 20 cases of MALT lymphoma. Expression of NF-kappaB was studied by immunostaining. Five cases of reactive lymphadenitis (RLA) were used as the control. Bcl10 rearrangement was detected in 8 of 20 (40{\%}) MALT lymphomas, but in none of RLA. Significant Bcl10 mutation was detected only in 1 case (5{\%}) with MALT, but not in RLA. RT-PCR showed higher density bands of Bcl10 in MALT lymphomas than in RLA. Immunostaining showed a weak Bcl10 expression in the germinal center and very weak expression in the marginal zone B-cells in RLA, which was limited to the cytoplasm. In contrast, Bcl10 was strongly expressed in MALT lymphomas, and was mainly detected in the cytoplasm, as well as in the nuclei. Bcl10 expression did not correlate with Bcl10 mutation and re-arrangements. NF-kappaB was expressed in nuclei of MALT lymphoma cells, but not in RLA. Bcl10 expression in MALT lymphoma correlated closely with NF-kappaB expression. Our results suggest that activation of Bcl10 and NF-kappaB may be important in MALT lymphomagenesis, and that nuclear localization of Bcl10 may be important in the progression of MALT.",
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AU - Ohshima, K.

AU - Muta, H.

AU - Kawasaki, C.

AU - Muta, K.

AU - Deyev, V.

AU - Kanda, M.

AU - Kumano, Y.

AU - Podack, E. R.

AU - Kikuchi, M.

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