BCL-2 improves oxidative phosphorylation and modulates adenine nucleotide translocation in mitochondria of cells harboring mutant mtDNA

Giovanni Manfredi, Jennifer Q. Kwong, José A. Oca-Cossio, Markus Woischnik, Carl D. Gajewski, Katherine Martushova, Marilena D'Aurelio, Avi L. Friedlichl, Carlos T Moraes

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Members of the BCL-2-related antiapoptotic family of proteins have been shown previously to regulate ATP/ADP exchange across the mitochondrial membranes and to prevent the loss of coupled mitochondrial respiration during apoptosis. We have found that BCL-2/BCL-xL can also improve mitochondrial oxidative phosphorylation in cells harboring pathogenic mutations in mitochondrial tRNA genes. The effect of BCL-2 overexpression in mutated cells was independent from apoptosis and was presumably associated with a modulation of adenine nucleotide exchange between mitochondria and cytosol. These results suggest that BCL-2 can regulate respiratory functions in response to mitochondrial distress by regulating the levels of adenine nucleotides.

Original languageEnglish
Pages (from-to)5639-5645
Number of pages7
JournalJournal of Biological Chemistry
Volume278
Issue number8
DOIs
StatePublished - Feb 21 2003

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Mitochondria
Adenine Nucleotides
Oxidative Phosphorylation
Mitochondrial DNA
Apoptosis
Mitochondrial Genes
Mitochondrial Membranes
Transfer RNA
Cytosol
Adenosine Diphosphate
Respiration
Genes
Adenosine Triphosphate
Modulation
Membranes
Mutation
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

BCL-2 improves oxidative phosphorylation and modulates adenine nucleotide translocation in mitochondria of cells harboring mutant mtDNA. / Manfredi, Giovanni; Kwong, Jennifer Q.; Oca-Cossio, José A.; Woischnik, Markus; Gajewski, Carl D.; Martushova, Katherine; D'Aurelio, Marilena; Friedlichl, Avi L.; Moraes, Carlos T.

In: Journal of Biological Chemistry, Vol. 278, No. 8, 21.02.2003, p. 5639-5645.

Research output: Contribution to journalArticle

Manfredi, G, Kwong, JQ, Oca-Cossio, JA, Woischnik, M, Gajewski, CD, Martushova, K, D'Aurelio, M, Friedlichl, AL & Moraes, CT 2003, 'BCL-2 improves oxidative phosphorylation and modulates adenine nucleotide translocation in mitochondria of cells harboring mutant mtDNA', Journal of Biological Chemistry, vol. 278, no. 8, pp. 5639-5645. https://doi.org/10.1074/jbc.M203080200
Manfredi, Giovanni ; Kwong, Jennifer Q. ; Oca-Cossio, José A. ; Woischnik, Markus ; Gajewski, Carl D. ; Martushova, Katherine ; D'Aurelio, Marilena ; Friedlichl, Avi L. ; Moraes, Carlos T. / BCL-2 improves oxidative phosphorylation and modulates adenine nucleotide translocation in mitochondria of cells harboring mutant mtDNA. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 8. pp. 5639-5645.
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