Basic fibroblast growth factor: A potential new therapeutic tool for the treatment of hypertrophic and keloid scars

Stephan Tiede, Nancy Ernst, Ardeshir Bayat, Ralf Paus, Volker Tronnier, Christina Zechel

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

Numerous tissue niches in the human body, such as skin, are now recognized to harbour adult stem cells. In this study, we analyze multipotent human dermis-derived progenitor cell populations, isolated and propagated from mechanically and enzymatically processed adult scalp skin. The populations encompass Nestin-positive and -negative cells, which may serve as a convenient and abundant source for various therapeutic applications in regenerative medicine. Here, we show that these cultures exhibit a strong tendency to differentiate into mesodermal derivatives, particularly myofibroblasts, when maintained in media containing serum. Since undesired and excessive myofibroblast formation is a frequent postsurgical complication, we sought culture conditions that would prevent myofibroblast formation. In particular, we analyzed the effect of growth factors, such as epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and platelet-derived growth factor AB (PDGF AB). Our results demonstrate that bFGF is a potent inhibitor of mesodermal differentiation, whereas PDFG AB favours myofibroblast formation and up-regulates expression of TGFβ receptors I and II. This interesting discovery may help in the prevention and treatment of tissue fibrosis and in particular in the eradication of hypertrophic and keloid scars.

Original languageEnglish (US)
Pages (from-to)33-44
Number of pages12
JournalAnnals of Anatomy
Volume191
Issue number1
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

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Keywords

  • Epidermal growth factor
  • Fibroblast growth factor
  • Hypertrophic scars
  • Keloid
  • Myofibroblast
  • Platelet-derived growth factor AB
  • Stem cells

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology

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