Abstract
Objective: To investigate BCP (basic calcium phosphate) crystal-stimulated mitogenesis and collagenase gene transcription in primary cultures of porcine chondrocytes. Methods. The role of protein kinases in BCP crystal-stimulated DNA synthesis was investigated using thymidine incorporation, kinase inhibitors, and protein kinase C (PKC) assays. Northern blot analysis was used to determine the levels of collagenase c-fos and c-jun message. Results. BCP crystals stimulated chondrocyte proliferation in a PKC-dependent manner. Increased levels of collagenase message were preceded by an increased accumulation of c-fos, but not c-jun. Conclusion. BCP crystals could contribute to the abnormal chondrocyte proliferation and collagenase secretion observed in some rheumatic diseases.
| Original language | English |
|---|---|
| Pages (from-to) | 343-350 |
| Number of pages | 8 |
| Journal | Arthritis and Rheumatism |
| Volume | 35 |
| Issue number | 3 |
| State | Published - Mar 1 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
- Rheumatology
Cite this
Basic calcium phosphate crystals stimulate cell proliferation and collagenase message accumulation in cultured adult articular chondrocytes. / Mitchell, Peter G.; Struve, Janine A.; McCarthy, Geraldine M.; Cheung, Herman S.
In: Arthritis and Rheumatism, Vol. 35, No. 3, 01.03.1992, p. 343-350.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Basic calcium phosphate crystals stimulate cell proliferation and collagenase message accumulation in cultured adult articular chondrocytes
AU - Mitchell, Peter G.
AU - Struve, Janine A.
AU - McCarthy, Geraldine M.
AU - Cheung, Herman S
PY - 1992/3/1
Y1 - 1992/3/1
N2 - Objective: To investigate BCP (basic calcium phosphate) crystal-stimulated mitogenesis and collagenase gene transcription in primary cultures of porcine chondrocytes. Methods. The role of protein kinases in BCP crystal-stimulated DNA synthesis was investigated using thymidine incorporation, kinase inhibitors, and protein kinase C (PKC) assays. Northern blot analysis was used to determine the levels of collagenase c-fos and c-jun message. Results. BCP crystals stimulated chondrocyte proliferation in a PKC-dependent manner. Increased levels of collagenase message were preceded by an increased accumulation of c-fos, but not c-jun. Conclusion. BCP crystals could contribute to the abnormal chondrocyte proliferation and collagenase secretion observed in some rheumatic diseases.
AB - Objective: To investigate BCP (basic calcium phosphate) crystal-stimulated mitogenesis and collagenase gene transcription in primary cultures of porcine chondrocytes. Methods. The role of protein kinases in BCP crystal-stimulated DNA synthesis was investigated using thymidine incorporation, kinase inhibitors, and protein kinase C (PKC) assays. Northern blot analysis was used to determine the levels of collagenase c-fos and c-jun message. Results. BCP crystals stimulated chondrocyte proliferation in a PKC-dependent manner. Increased levels of collagenase message were preceded by an increased accumulation of c-fos, but not c-jun. Conclusion. BCP crystals could contribute to the abnormal chondrocyte proliferation and collagenase secretion observed in some rheumatic diseases.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026609337&partnerID=8YFLogxK
M3 - Article
C2 - 1311178
AN - SCOPUS:0026609337
VL - 35
SP - 343
EP - 350
JO - Arthritis and Rheumatology
JF - Arthritis and Rheumatology
SN - 2326-5191
IS - 3
ER -