Basic calcium phosphate crystals stimulate cell proliferation and collagenase message accumulation in cultured adult articular chondrocytes

Peter G. Mitchell, Janine A. Struve, Geraldine M. McCarthy, Herman S Cheung

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Objective: To investigate BCP (basic calcium phosphate) crystal-stimulated mitogenesis and collagenase gene transcription in primary cultures of porcine chondrocytes. Methods. The role of protein kinases in BCP crystal-stimulated DNA synthesis was investigated using thymidine incorporation, kinase inhibitors, and protein kinase C (PKC) assays. Northern blot analysis was used to determine the levels of collagenase c-fos and c-jun message. Results. BCP crystals stimulated chondrocyte proliferation in a PKC-dependent manner. Increased levels of collagenase message were preceded by an increased accumulation of c-fos, but not c-jun. Conclusion. BCP crystals could contribute to the abnormal chondrocyte proliferation and collagenase secretion observed in some rheumatic diseases.

Original languageEnglish
Pages (from-to)343-350
Number of pages8
JournalArthritis and Rheumatism
Volume35
Issue number3
StatePublished - Mar 1 1992
Externally publishedYes

Fingerprint

Collagenases
Chondrocytes
Joints
Cell Proliferation
Protein Kinase C
Thymidine Kinase
Rheumatic Diseases
Northern Blotting
Protein Kinases
Swine
calcium phosphate
DNA
Genes

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Basic calcium phosphate crystals stimulate cell proliferation and collagenase message accumulation in cultured adult articular chondrocytes. / Mitchell, Peter G.; Struve, Janine A.; McCarthy, Geraldine M.; Cheung, Herman S.

In: Arthritis and Rheumatism, Vol. 35, No. 3, 01.03.1992, p. 343-350.

Research output: Contribution to journalArticle

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