Basic calcium phosphate crystal-induced Egr-1 expression stimulates mitogenesis in human fibroblasts

Xiao R. Zeng, Yubo Sun, Leonor Wenger, Herman S. Cheung

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Previously, we have reported that basic calcium phosphate (BCP) crystals stimulate mitogenesis and synthesis of matrix metalloproteinases in cultured human foreskin and synovial fibroblasts. However, the detailed mechanisms involved are still unclear. In the present study, using RT-PCR and Egr-1 promoter analysis we showed that BCP crystals could stimulate early growth response gene Egr-1 transcription through a PKCα-dependent p44/p42 MAPK pathway. Using a retrovirus gene expression system (Clontech) to overexpress Egr-1 in human fibroblast BJ-1 cells resulted in promotion of mitogenesis measured either by MTT cell proliferation analysis or by direct cell counting. The results demonstrate that Egr-1 may play a key role in mediating BCP crystal-induced synovial fibroblast mitogenesis.

Original languageEnglish (US)
Pages (from-to)658-664
Number of pages7
JournalBiochemical and biophysical research communications
Volume330
Issue number3
DOIs
StatePublished - May 13 2005

Keywords

  • BCP crystals
  • Egr-1
  • Fibroblasts
  • Mitogenesis
  • Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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